Questions and answers for exam Preparation

MCQs

Total Questions : 72 | Page 4 of 8 pages

Question 31. How can PCR product be cloned into a vector?

It can be done only when PCR products are blunt-ended
It can be done only by restriction enzyme digestion
Both the methods can be used
The blunt-ended approach is favoured

Discuss Question

Answer: Option B. -> It can be done only by restriction enzyme digestion
Answer: (b).It can be done only by restriction enzyme digestion

Question 32. Which of the following statement is incorrect regarding the cloning of PCR products?

In cloning via restriction enzymes, restriction sites are induced before amplification is carried out
The restriction sites are induced in the primers before annealing
The intermediate molecules are having restriction sites at both ends
The amplified molecules can be cut at both the ends by appropriate enzymes

Discuss Question

Answer: Option C. -> The intermediate molecules are having restriction sites at both ends
Answer: (c).The intermediate molecules are having restriction sites at both ends

Question 33. Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?

The restriction site is induced into the vector and the topisomerase enzyme is induced into the PCR primers
The topoismerase I is used for cutting both the strands
The induction of topoisomerase enzyme is done into the vector in the case it is very small in size
The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector

Discuss Question

Answer: Option D. -> The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector
Answer: (d).The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector

Question 34. Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________

Inverse PCR
Circular PCR
Non-conventional PCR
In-situ PCR

Discuss Question

Answer: Option A. -> Inverse PCR
Answer: (a).Inverse PCR

Question 35. Which one of the following is not done if amplification of the non flanking region is carried out?

Firstly, restriction enzyme digestion is done of those sites whose sequence is not known
Then the self-ligation of molecules is allowed
Now the molecules are cleaved where the known sequence is
Again, the molecules are linearized and the known sequence is in the middle

Discuss Question

Answer: Option D. -> Again, the molecules are linearized and the known sequence is in the middle
Answer: (d).Again, the molecules are linearized and the known sequence is in the middle

Question 36. Reverse transciptase PCR is also carried out at times. Which of the statement is true?

Amplification of RNA samples is not required for knowing the abundance of mRNA
Both the start and the end primers are used
Only a single cDNA strand is synthesized before the PCR
The primer used is always specific

Discuss Question

Answer: Option D. -> The primer used is always specific
Answer: (d).The primer used is always specific

Question 37. How many approaches are there for measuring the quantity of PCR products?

Discuss Question

Answer: Option B. -> 2
Answer: (b).2

Question 38. Which type of PCR allows the use of permeabilized tissue?

Inverse PCR
In-situ PCR
Quantitative PCR
Hot-start PCR

Discuss Question

Answer: Option B. -> In-situ PCR
Answer: (b).In-situ PCR

Question 39. Which of the statement hold true for Quantitative PCR?

A fluorescent dye is used which binds on single stranded DNA molecules
SYBR green is an example such type of dye
The quantity of DNA is simply measured by measuring the amount of fluorescence
This approach is useful if the products are non-specific in nature

Discuss Question

Answer: Option C. -> The quantity of DNA is simply measured by measuring the amount of fluorescence
Answer: (c).The quantity of DNA is simply measured by measuring the amount of fluorescence

Question 40. In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

It allows detection of all double stranded molecules
The reporter and quencher are the molecules present on the same probe
The quencher is having a fluorescent group
Fluorescence is observed only when both the groups are present in proximity to each other

Discuss Question

Answer: Option B. -> The reporter and quencher are the molecules present on the same probe
Answer: (b).The reporter and quencher are the molecules present on the same probe