Questions and answers for exam Preparation

MCQs

Total Questions : 72 | Page 7 of 8 pages

Question 61. Isothermal amplification is carried out at times. Which of the statement is true?

The repeated heating and cooling required by PCR is not the reason for limiting how quickly the reaction is carried out
It is carried out typically at 65 degrees
Normal Taq polymerase is used
It requires expensive PCR machines to be carried out

Discuss Question

Answer: Option B. -> It is carried out typically at 65 degrees
Answer: (b).It is carried out typically at 65 degrees

Question 62. Emulsion or droplet PCR is another modification of PCR. Which of the statement holds true?

It is possible to incorporate the reagents in a lipid drop
It refers to the PCR carried out at large scale
The temperature variation is not possible easily
If there is a single template molecule at the start then amplification results in the mixture and it because of the extent of extension carried out

Discuss Question

Answer: Option A. -> It is possible to incorporate the reagents in a lipid drop
Answer: (a).It is possible to incorporate the reagents in a lipid drop

Question 63. What is the half life cycle for Taq polymerase?

40 minutes
80 minutes
10 minutes
50 minutes

Discuss Question

Answer: Option A. -> 40 minutes
Answer: (a).40 minutes

Question 64. Polymerases are also available from other Thermus species. Which of the following is correct?

Thermus flavus gives Tfl enzyme
Thermus thermopilus gives Tfl enzyme
They are having proof reading activity
Thermus flavus gives Tth enzyme

Discuss Question

Answer: Option A. -> Thermus flavus gives Tfl enzyme
Answer: (a).Thermus flavus gives Tfl enzyme

Question 65. Taq polymerase incorporates which residue at 3’ end?

Discuss Question

Answer: Option C. -> A
Answer: (c).A

Question 66. Thermococcus litoralis grows at a temperature upto 98 degrees.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 67. Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?

They add A residue at 3’ end
They are obtained from Thermococcus litoralis
They can’t be obtained from archaebacteria
The marine bacteria from which they are obtained grow at temperatures lower than that of Thermus aquatics

Discuss Question

Answer: Option B. -> They are obtained from Thermococcus litoralis
Answer: (b).They are obtained from Thermococcus litoralis

Question 68. During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?

Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus
Direct sequencing can be used if the template DNA is heterozygous at the locus
If cloning is done before sequencing, then it is detected via using only a single clone for sequencing
In the case several recombinants are used, it can’t go undetected

Discuss Question

Answer: Option B. -> Direct sequencing can be used if the template DNA is heterozygous at the locus
Answer: (b).Direct sequencing can be used if the template DNA is heterozygous at the locus

Question 69. If the template DNA belongs to several individual rather than single one, this type of heterogeneity is known as ____________

Heterzygosity
Product heterogeneity
Population heterogeneity
Template heterogeneity

Discuss Question

Answer: Option C. -> Population heterogeneity
Answer: (c).Population heterogeneity

Question 70. Which of the statement is correct for misincorporation?

If direct sequencing has carried this misincorporation is a great problem
The erroneous molecules give strong signals than genuine molecules in case of misincorporation
If the misincorporation in cloned PCR products it is a problem
Even if the error is induced at an early stage it is not incorporated in many sequences

Discuss Question

Answer: Option C. -> If the misincorporation in cloned PCR products it is a problem
Answer: (c).If the misincorporation in cloned PCR products it is a problem