Questions and answers for exam Preparation

MCQs

Total Questions : 72 | Page 5 of 8 pages

Question 41. Primers are generally ____________

20-30 nucleotides long
40-50 nucleotides long
as long as the template is
taken according to the amount available

Discuss Question

Answer: Option A. -> 20-30 nucleotides long
Answer: (a).20-30 nucleotides long

Question 42. Which end of the primer should be matched properly in order to carry out the amplification?

5’ end
3’ end
Both of the ends should be matched properly
Anyone of the ends should match

Discuss Question

Answer: Option B. -> 3’ end
Answer: (b).3’ end

Question 43. Melting temperature is given by ____________

4(G+C) + 2(A+T)
2(G+C) + 4(A+T)
2(A+G) + 4(C+T)
4(A+G) + 2(C+T)

Discuss Question

Answer: Option A. -> 4(G+C) + 2(A+T)
Answer: (a).4(G+C) + 2(A+T)

Question 44. Both the primers, the start primer and the end primer should have a nearly same melting temperature.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 45. Which of the following nucleotides should be there at 3’ end?

Any of A, T, G or C will work out
Either A or T
Either G or C
Specifically G

Discuss Question

Answer: Option C. -> Either G or C
Answer: (c).Either G or C

Question 46. In the case of uncertainty, if more than one nucleotide is included at a position it is called ____________

mixed site
polynucleotide site
unique site
degenerate site

Discuss Question

Answer: Option A. -> mixed site
Answer: (a).mixed site

Question 47. What is the property of inosine?

Having narrow range of pairing capabilities
Having a broad range of pairing capabilities
The pairing capability is the same as the normal nucleotides
It is abbreviated as A

Discuss Question

Answer: Option B. -> Having a broad range of pairing capabilities
Answer: (b).Having a broad range of pairing capabilities

Question 48. Why internal secondary structures are not preferred for primers?

Internal structures are very bulky and thus elongation is not preferred
Because of it, primer may fold back on itself and won’t be available for template
Internal secondary structures require more amount of template
If internal structures are present, no proof reading would be observed

Discuss Question

Answer: Option B. -> Because of it, primer may fold back on itself and won’t be available for template
Answer: (b).Because of it, primer may fold back on itself and won’t be available for template

Question 49. What will happen if the amino acid sequence is used directly for primer designing?

There would be certainty because the genetic code is unique for each amino acid
There would be uncertainty as the genetic code is degenerate and none of the amino acid is having a unique code
There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon
The amount of uncertainty or certainty is a matter of chance

Discuss Question

Answer: Option C. -> There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon
Answer: (c).There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon

Question 50. Which of the following is favoured for primer design?