Questions and answers for exam Preparation

MCQs

Total Questions : 72 | Page 6 of 8 pages

Question 51. Which of the following is useful in applications of PCR?

It is manual
Only one sample’s analysis can be carried out at a time
It is having a high speed
The amount of DNA required initially is high

Discuss Question

Answer: Option C. -> It is having a high speed
Answer: (c).It is having a high speed

Question 52. What is the correct statement with respect to ddNTPs?

They are dideoxynucleotide triphosphates
They are used in the termination of DNA sequencing
They are used for initiating DNA sequencing
They are used in the case if the starting amounts are large

Discuss Question

Answer: Option B. -> They are used in the termination of DNA sequencing
Answer: (b).They are used in the termination of DNA sequencing

Question 53. Cycle sequencing is the DNA sequencing where very less amounts of template are utilised for carrying out the sequencing.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 54. PCR products can be analysed in many ways. Which of the following is not possible?

Use of restriction enzymes
Determining whether a particular oliginucleotide probe hybridizes to a PCR product
Electrophoresis
Direct sequencing can’t be carried out

Discuss Question

Answer: Option D. -> Direct sequencing can’t be carried out
Answer: (d).Direct sequencing can’t be carried out

Question 55. Sickle cell anaemia is a genetic disorder. Which of the following doesn’t holds true for it?

It can be analysed by PCR
It destroys a restriction site
The mutation is in alpha globulin gene
The conventional approach took weeks for the whole analyses to be carried out

Discuss Question

Answer: Option C. -> The mutation is in alpha globulin gene
Answer: (c).The mutation is in alpha globulin gene

Question 56. Which of the statements don’t hold true for the forensics and the amplification carried out?

In the case of forensics, conventional methods such as southern blotting are used very effectively
In cases of bone fragments which contain less than 300 nucleotides conventional methods can’t be applied as they involve southern blotting, restriction digestion etc
The poor condition of DNA also makes the PCR amplification difficult
Microsatellites composed of simply varying repeats of CA sequences are used

Discuss Question

Answer: Option A. -> In the case of forensics, conventional methods such as southern blotting are used very effectively
Answer: (a).In the case of forensics, conventional methods such as southern blotting are used very effectively

Question 57. PCR is useful in population genetics because at times it can be used to study genetics of bacteria that can’t be cultured axenically.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 58. What is the problem associated with historical DNA samples?

They are less in amount thus amplification is difficult
Because the samples are very old, there can be contamination
They degrade during repeated cooling and heating cycles
As the samples are old, the standard sequences for comparison is not present

Discuss Question

Answer: Option B. -> Because the samples are very old, there can be contamination
Answer: (b).Because the samples are very old, there can be contamination

Question 59. The genetic relatedness between organisms can be identified by studying the band patterns when different PCR products are analysed electrophoreically. This method is called as ____________