Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning? Options: A . &nbspThe restriction site is induced into the vector and the topisomerase enzyme is induced into the PCR primers B . &nbspThe topoismerase I is used for cutting both the strands C . &nbspThe induction of topoisomerase enzyme is done into the vector in the case it is very small in size D . &nbspThe restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector

Topoisomerse I is also used for cloning of PCR product at times. Which of the st

Question

Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?

Options:

A . The restriction site is induced into the vector and the topisomerase enzyme is induced into the PCR primers

B . The topoismerase I is used for cutting both the strands

C . The induction of topoisomerase enzyme is done into the vector in the case it is very small in size

D . The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector

Answer: Option D
Answer: (d).The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector

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Question 1. Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers....carried out outside the primers, it is called as __________

Question 2. Which one of the following is not done if amplification of the non flanking region is carried out?....

Question 3. Reverse transciptase PCR is also carried out at times. Which of the statement is true?....

Question 4. How many approaches are there for measuring the quantity of PCR products?....

Question 5. Which type of PCR allows the use of permeabilized tissue?....

Question 6. Which of the statement hold true for Quantitative PCR?....

Question 7. In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this meth....used. Which of the statement holds true for this methodology?

Question 8. Primers are generally ____________....

Question 9. Which end of the primer should be matched properly in order to carry out the amplification?....

Question 10. Melting temperature is given by ____________....

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