Questions and answers for exam Preparation

MCQs

Total Questions : 72 | Page 2 of 8 pages

Question 11. Which can be used as a precaution in order to minimize contamination?

Careful use and design of pipettes
Placing the pre-PCR and post-PCR stages in the same rooms
Extracting the DNA along with surface layers
Use of primers carefully is not very important

Discuss Question

Answer: Option A. -> Careful use and design of pipettes
Answer: (a).Careful use and design of pipettes

Question 12. How can the specificity of primer annealing be increased?

Use of short primers
Raising temperature
Adjusting the concentration of sodium ions
Using polymerase with proof reading activity

Discuss Question

Answer: Option B. -> Raising temperature
Answer: (b).Raising temperature

Question 13. There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 14. Which of the statement holds for long-range PCR and in its relation?

It is the PCR in which longer templates are used
DNA polymerases which don’t have proof-reading activity give larger products
DNA polymerases’ processivity is not a measure to have larger products
It is PCR in which a mixture of enzymes is used to have larger products

Discuss Question

Answer: Option D. -> It is PCR in which a mixture of enzymes is used to have larger products
Answer: (d).It is PCR in which a mixture of enzymes is used to have larger products

Question 15. Which of the following conditions don’t contribute to wrong annealing to primer?

Chance complementarity
Conditions of annealing
The original sequence of the primers
Both the conditions of annealing and the original sequence don’t play any role

Discuss Question

Answer: Option D. -> Both the conditions of annealing and the original sequence don’t play any role
Answer: (d).Both the conditions of annealing and the original sequence don’t play any role

Question 16. The process of amplification of specific DNA sequences by an enzymatic process is termed as ____________

amplification
polymerase chain reaction (PCR)
translation
microarrays

Discuss Question

Answer: Option A. -> amplification
Answer: (a).amplification

Question 17. These are steps taken in carrying out the PCR reaction:
i) Attaching of primers by cooling
ii) Denaturation of strands
iii) DNA synthesis
iv) Heating
Which is the correct order?
(Mentioned from starting to ending the reaction)

i)-ii)-iii)-iv)
ii)-i)-iii)-iv)
iv)-iii)-ii)-i)
iv)-ii)-i)-iii)

Discuss Question

Answer: Option D. -> iv)-ii)-i)-iii)
Answer: (d).iv)-ii)-i)-iii)

Question 18. What are primers?

Primers are the short sequences at the end of the nucleotide sequences which are used for amplification
Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified
Primers are the short sequences present anywhere in the nucleotide sequence to be amplified
Primers are the short sequences which are complementary to the nucleotides anywhere in the sequence to be amplified

Discuss Question

Answer: Option B. -> Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified
Answer: (b).Primers are the short sequences which are complementary to the nucleotides at the end of the sequence which is to be amplified

Question 19. A reaction mixture for PCR consists of ____________

heat unstable polymerase
primers in a limited amount
deoxynucleoside triphosphate (dNTPs)
a region complementary to the sequence to be amplified

Discuss Question

Answer: Option C. -> deoxynucleoside triphosphate (dNTPs)
Answer: (c).deoxynucleoside triphosphate (dNTPs)

Question 20. Which of the following is a characteristic of Taq polymerase?

It is an RNA polymerase
It is heat stable
It is obtained from thermophilic bacterium and can be grown in the laboratory below a temperature of 75 degrees
It is used in cellular synthesis processes and the optimum temperature is at least 90 degrees

Discuss Question

Answer: Option B. -> It is heat stable
Answer: (b).It is heat stable