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Question
Which one of the following is not done if amplification of the non flanking region is carried out?
Options:
A .  Firstly, restriction enzyme digestion is done of those sites whose sequence is not known
B .  Then the self-ligation of molecules is allowed
C .  Now the molecules are cleaved where the known sequence is
D .  Again, the molecules are linearized and the known sequence is in the middle
Answer: Option D
Answer: (d).Again, the molecules are linearized and the known sequence is in the middle

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