Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 7 of 12 pages

Question 61. Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

Prophase
Telophase
Metaphase
Anaphase

Discuss Question

Answer: Option C. -> Metaphase
Answer: (c).Metaphase

Question 62. The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________

microdissection
chromosome sorting
chromosome walking
chromosome jumping

Discuss Question

Answer: Option A. -> microdissection
Answer: (a).microdissection

Question 63. If the library to be screened and the host belongs to different species, then it is called as ___________

homologous selection
heterologous selection
intraspecies selection
mixed selection

Discuss Question

Answer: Option B. -> heterologous selection
Answer: (b).heterologous selection

Question 64. For cloning purposes, the intact chromosomes should be separated by ___________

agarose gel electrophoresis
fluorescence- activated sorter
polyacrylamide gel electrophoresis
chromatography

Discuss Question

Answer: Option B. -> fluorescence- activated sorter
Answer: (b).fluorescence- activated sorter

Question 65. The direct selection allows _______ number of recombinants to be screened and _______

less, slowly
less, quickly
more, quickly
more, slowly

Discuss Question

Answer: Option C. -> more, quickly
Answer: (c).more, quickly

Question 66. Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes
The RNA is translated within oocytes
Screening is carried out on the basis of DNA
The screening is helpful for the proteins whose function can be easily screened such as transport of ions

Discuss Question

Answer: Option C. -> Screening is carried out on the basis of DNA
Answer: (c).Screening is carried out on the basis of DNA

Question 67. In the case of ligand binding by the expressed protein, the library can be screened by ______________

In the case of ligand binding by the expressed protein, the library can be screened by ______________
ligands if the sequence we are looking for encodes a protein specific to a ligand
using a specific DNA sequence which can bind to the protein encoded by the sequence of interest
all of the mentioned

Discuss Question

Answer: Option D. -> all of the mentioned
Answer: (d).all of the mentioned

Question 68. Choose the incorrect statement for panning.

It is a variant of ligand binding approach
The ligand is immobilized on the solid support and the cells are passed over it
If the ligand-binding domain is exposed on surface of the any recombinant molecules then they will bind to ligand
They are not useful for scanning libraries in cultured mammalian cells

Discuss Question

Answer: Option D. -> They are not useful for scanning libraries in cultured mammalian cells
Answer: (d).They are not useful for scanning libraries in cultured mammalian cells

Question 69. The ligand should bind to a single protein only for the screening process.