Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 9 of 12 pages

Question 81. Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them.

Nitrocellulose membranes were preferred earlier
They bind DNA very efficiently
They can be handled easily without breakage
They are inflammable

Discuss Question

Answer: Option C. -> They can be handled easily without breakage
Answer: (c).They can be handled easily without breakage

Question 82. Choose the incorrect statement for prehybridization mixture.

The function of it is to carry out specific binding
It constitutes of non-labelled DNA of non-specific sequence which is obtained from salmon or herring testes or sperm
It is added in a small amount
It is carried out before the hybridization is carried out

Discuss Question

Answer: Option A. -> The function of it is to carry out specific binding
Answer: (a).The function of it is to carry out specific binding

Question 83. What is used for lysing of bacterial cells and denaturation of DNA?

Exonuclease
Sulphuric Acid
Sodium Hydroxide
Heat

Discuss Question

Answer: Option C. -> Sodium Hydroxide
Answer: (c).Sodium Hydroxide

Question 84. The process of baking or cross-linking of the membrane is carried out by ___________

By heating at 80 degrees
By irradiating with UV
Both irradiation and heating method are used
By addition of agarose

Discuss Question

Answer: Option C. -> Both irradiation and heating method are used
Answer: (c).Both irradiation and heating method are used

Question 85. Nitrocellulose membranes are less sensitive than nylon membranes.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 86. After hybridization how the position of the labelled DNA probe is determined?

By staining
By UV irradiation
By using chemiluminescence
By electronic microscope

Discuss Question

Answer: Option C. -> By using chemiluminescence
Answer: (c).By using chemiluminescence

Question 87. The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________

Size of the probe
Proportion of only A
Ionic concentration of hybridization buffer
Other agents which alter the base pairing

Discuss Question

Answer: Option B. -> Proportion of only A
Answer: (b).Proportion of only A

Question 88. If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

PCR amplification of the probe can be carried out
Excision of the probe should be carried out which should be followed polyacrylamide gel electrophoresis
If the vector sequence and the DNA probe are having similarities, some of the vector would be hybridized
Probe should be in a vector that is having some sequence similarity with the library to be screened

Discuss Question

Answer: Option A. -> PCR amplification of the probe can be carried out
Answer: (a).PCR amplification of the probe can be carried out

Question 89. At what temperatures the hybridization and washing of the DNA probes should be carried out?