Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 6 of 12 pages

Question 51. Choose the incorrect statement for the homopolymer tailing of cDNA strands.

Choose the incorrect statement for the homopolymer tailing of cDNA strands.
Vector is also treated with terminal transferase and dGTPs
The vector and cDNA can now anneal with the help of DNA ligase
If gaps are created they can be repaired by physiological processes

Discuss Question

Answer: Option C. -> The vector and cDNA can now anneal with the help of DNA ligase
Answer: (c).The vector and cDNA can now anneal with the help of DNA ligase

Question 52. In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

library
shelf
small library
mini library

Discuss Question

Answer: Option B. -> shelf
Answer: (b).shelf

Question 53. Any cDNA library would represent a fraction of RNA species of an organism.

True
False
May be True or False
Can't Say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 54. Choose the correct statement for construction of a library subsection.

The size of a particular restriction fragment on which the gene is located is not known
The size of the restriction fragment can be known by carrying out southern blotting
Another digest of the genomic DNA is carried out by a different enzyme
DNA fragments of different size are recovered after carrying out gel electrophoresis

Discuss Question

Answer: Option B. -> The size of the restriction fragment can be known by carrying out southern blotting
Answer: (b).The size of the restriction fragment can be known by carrying out southern blotting

Question 55. Choose the correct statement if the RNA is non polydenylated.

A collection of chemically synthesized oligonucleotides is used as primers
They are usually tetramer
Unequal quantities of A, G, T and C are used
The primers attach at only specific sequences for first strand synthesis

Discuss Question

Answer: Option A. -> A collection of chemically synthesized oligonucleotides is used as primers
Answer: (a).A collection of chemically synthesized oligonucleotides is used as primers

Question 56. What do we mean by housekeeping genes?

Housekeeping genes are those genes which are specific to an organism
Housekeeping genes are those genes which are present in all the organisms
Housekeeping genes are those genes which are meant for repair and maintenance in a species of organism
Housekeeping genes are those genes which required for the replication process

Discuss Question

Answer: Option B. -> Housekeeping genes are those genes which are present in all the organisms
Answer: (b).Housekeeping genes are those genes which are present in all the organisms

Question 57. What is done after the recovery of pellets has been carried out in order to know the amount of polypeptides?

Denaturing and gel electrophoresis in SDS- Polyacryamide gel
Gel electrophoresis in agarose gel
Quantitative PCR
Weighing pellets

Discuss Question

Answer: Option A. -> Denaturing and gel electrophoresis in SDS- Polyacryamide gel
Answer: (a).Denaturing and gel electrophoresis in SDS- Polyacryamide gel

Question 58. The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction
Along with simple antigen-antibody complex, a substrate is added for easy precipitation
Protein A-Sepharose is added
Protein A-Sepharose binds to IgG antibodies

Discuss Question

Answer: Option A. -> Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction
Answer: (a).Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

Question 59. Choose the correct statement for RNA fractionation.

The RNA is fractioned by size but before separating on oligo-dT cellulose
A sucrose density gradient is used
The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster
Different bands are formed according to the density in the sucrose density gradient

Discuss Question

Answer: Option B. -> A sucrose density gradient is used
Answer: (b).A sucrose density gradient is used

Question 60. What is done after RNA fractionation is carried out?

Each band is translated in vivo
Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells
Translation is carried out with a high background
Amino acid is not radioactively labelled

Discuss Question

Answer: Option B. -> Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells
Answer: (b).Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells