Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 11 of 12 pages

Question 101. Independent assortment of genes and their corresponding traits were first discovered by

Darwin
Oscar Hertwig
Gregor Mendel
Walther Flemming

Discuss Question

Answer: Option C. -> Gregor Mendel
Answer: (c).Gregor Mendel

Question 102. In case of induced fit model

the substrate change its shape slightly
the enzyme change its shape slightly
none of them changes shape
both changes shapes

Discuss Question

Answer: Option B. -> the enzyme change its shape slightly
Answer: (b).the enzyme change its shape slightly

Question 103. According to lock and key model the substrate acts as a

key
lock
Inhibitor
Enzyme

Discuss Question

Answer: Option A. -> key
Answer: (a).key

Question 104. If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________

nascent peptide display
mRNA display
ribosome display
ribozyme display

Discuss Question

Answer: Option C. -> ribosome display
Answer: (c).ribosome display

Question 105. Libraries can broadly be classified into how many types?

Discuss Question

Answer: Option B. -> 2
Answer: (b).2

Question 106. Choose the correct statement for genomic libraries.

Genomic libraries include the representation of the whole genome of the organism
Sequences such as telomeres are also represented
Telomeres can be readily cloned
The larger the size of the insert of genomic DNA in recombinants, the more is the number of recombinants required to represent the genome in the library

Discuss Question

Answer: Option A. -> Genomic libraries include the representation of the whole genome of the organism
Answer: (a).Genomic libraries include the representation of the whole genome of the organism

Question 107. Choose the incorrect statement for the preparation of genomic libraries.

The first step is the isolation of genomic DNA
Physical damage to the DNA should be avoided
If a nuclear DNA library is to be constructed, organelle DNA is to be removed
For the construction of organelle library, organelle DNA is purified from the nuclear DNA

Discuss Question

Answer: Option C. -> If a nuclear DNA library is to be constructed, organelle DNA is to be removed
Answer: (c).If a nuclear DNA library is to be constructed, organelle DNA is to be removed

Question 108. The various steps for construction of libraries are ___________
i) Fragmentation of DNA
ii) Isolation of genomic DNA
iii) Amplification
iv) Ligation and introduction to the host
v) Vector preparation
The correct order of construction of libraries is (In the order of starting to ending).

i)-ii)-iii)-iv)-v)
ii)-i)-v)-iv)-iii)
ii)-v)-i)-iv)-iii)
v)-ii)-i)-iii)-iv)

Discuss Question

Answer: Option B. -> ii)-i)-v)-iv)-iii)
Answer: (b).ii)-i)-v)-iv)-iii)

Question 109. Poly A tail from the column is eluted by using high salt concentration.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 110. Choose the incorrect statement for second DNA strand synthesis.

The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol
The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends
These RNA fragments can be used as primers
There is no portion of RNA left attached to the DNA strand

Discuss Question

Answer: Option D. -> There is no portion of RNA left attached to the DNA strand
Answer: (d).There is no portion of RNA left attached to the DNA strand