Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 2 of 12 pages

Question 11. If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 12. If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called ___________

in-silico
homologue search
annotation
partial search

Discuss Question

Answer: Option C. -> annotation
Answer: (c).annotation

Question 13. How many techniques are there for carrying out the screening of sequences encoding for RNAs?

Discuss Question

Answer: Option B. -> 2
Answer: (b).2

Question 14. The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:
i) Peeling of membranes carrying away bacterial cells with it
ii) Cells are lysed and denaturing of DNA is being carried out
iii) Hybridization with labelled DNA
iv) Placing the membrane onto plate containing recombinant cells
Choose the correct sequence in which the steps are carried out (starting to ending).

iv)-i)-ii)-iii)
iv)-ii)-i)-iii)
i)-ii)-iii)-iv)
iii)-ii)-i)-iv)

Discuss Question

Answer: Option A. -> iv)-i)-ii)-iii)
Answer: (a).iv)-i)-ii)-iii)

Question 15. Choose the incorrect statement for colony or plaque lift.

It is the base for screening based on nucleic acid hybridization
It is also known as Grunstein-Hogness technique
It is based on the fact that which members of the library have the same sequence as the DNA probe
The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

Discuss Question

Answer: Option C. -> It is based on the fact that which members of the library have the same sequence as the DNA probe
Answer: (c).It is based on the fact that which members of the library have the same sequence as the DNA probe

Question 16. Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________

chromosome jumping
chromosome sorting
chromosome walking
transposon tagging

Discuss Question

Answer: Option C. -> chromosome walking
Answer: (c).chromosome walking

Question 17. Mobile portions of a chromosome which can be transferred from one portion to another are termed as ___________

mobile part
transposons
walking elements
transferrable genetic element

Discuss Question

Answer: Option B. -> transposons
Answer: (b).transposons

Question 18. If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________

locational cloning
positional cloning
chromosome walking
transposon tagging

Discuss Question

Answer: Option B. -> positional cloning
Answer: (b).positional cloning

Question 19. At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this?

Discuss Question

Answer: Option B. -> 2
Answer: (b).2

Question 20. Choose the correct statement if the screening is carried out by screening by expression in vivo.

The proportion of recombinants carrying gene of interest is small
The recombinants carrying gene of interest don’t complement the host mutation
Mutation should be affecting many genes
Most of the products selected would be result of complementation

Discuss Question

Answer: Option A. -> The proportion of recombinants carrying gene of interest is small
Answer: (a).The proportion of recombinants carrying gene of interest is small