Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 5 of 12 pages

Question 41. For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?

It is so because it is unstable
It is so because it is at the 3’ end of the template molecule
It is so because it would be unable to initiate replication
It is so because it detaches itself after first strand synthesis

Discuss Question

Answer: Option C. -> It is so because it would be unable to initiate replication
Answer: (c).It is so because it would be unable to initiate replication

Question 42. Choose the correct with respect to RNA molecules.

They are less labile than DNA molecules
The 2’ hydroxyl group of ribose group decreases the activity
There is no less of activity while boiling
Baking of glassware, treatment with UV can be used for protection against degradation

Discuss Question

Answer: Option D. -> Baking of glassware, treatment with UV can be used for protection against degradation
Answer: (d).Baking of glassware, treatment with UV can be used for protection against degradation

Question 43. The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA.

True
False
May be True or False
Can't Say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 44. At times streptavidin is used in place of cellulose. Choose the correct statement for this alternative.

oligo-dT streptavidin conjugate can be extracted by magnetic beads
The magnetic beads are attached with activin
Recovery is not based on the magnetic properties of the beads
The interaction between polyA tail and the oligo-dT is reduced

Discuss Question

Answer: Option A. -> oligo-dT streptavidin conjugate can be extracted by magnetic beads
Answer: (a).oligo-dT streptavidin conjugate can be extracted by magnetic beads

Question 45. What is the basis of RNaseH method?

It is based on RNA synthesis by DNA strand
It is based on complementary DNA synthesis by RNA strand through reverse transcriptase
It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme
It is based on getting double strand RNA from a single strand

Discuss Question

Answer: Option B. -> It is based on complementary DNA synthesis by RNA strand through reverse transcriptase
Answer: (b).It is based on complementary DNA synthesis by RNA strand through reverse transcriptase

Question 46. Choose the incorrect statement with respect to host used.

The host must carry mutation for the gene of interest
The host should not contain restriction enzymes
The host if having restriction enzymes, then the incoming DNA should not be methylated
The host should be deficient in recombination

Discuss Question

Answer: Option C. -> The host if having restriction enzymes, then the incoming DNA should not be methylated
Answer: (c).The host if having restriction enzymes, then the incoming DNA should not be methylated

Question 47. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______

coupled, linked
linked, coupled
grouped, separate
grouped, individual

Discuss Question

Answer: Option A. -> coupled, linked
Answer: (a).coupled, linked

Question 48. If translation and transcription are carried out separately, then what is added to carry out translation?

DNA polymerase
RNA polymerase
Taq polymerase
Tfl polymerase

Discuss Question

Answer: Option B. -> RNA polymerase
Answer: (b).RNA polymerase

Question 49. If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 50. If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

The extract in which the host DNA constructs are incubated is capable of translation only
The extract in which the host DNA constructs are incubated is capable of transcription only
The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc
Non radiolabelled amino acids are used

Discuss Question

Answer: Option C. -> The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc
Answer: (c).The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc