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MCQs

Total Questions : 113 | Page 8 of 12 pages
Question 71. Choose the correct statement with respect to the self priming method of cDNA synthesis.
  1.    It is less preferred than RNaseH method
  2.    A hairpin structure is formed with guarantee
  3.    The sequence corresponding to the 5’ end is lost
  4.    Reverse transcriptase is not used
 Discuss Question
Answer: Option C. -> The sequence corresponding to the 5’ end is lost
Answer: (c).The sequence corresponding to the 5’ end is lost
Question 72. Choose the correct statement for RACE.
  1.    It stands for Random Amplification of cDNA ends
  2.    It is for cloning particular cDNA ends
  3.    It is only of one type, which is 5’ RACE
  4.    Sequence data is not available in any case
 Discuss Question
Answer: Option B. -> It is for cloning particular cDNA ends
Answer: (b).It is for cloning particular cDNA ends
Question 73. Choose the incorrect statement for the method homopolymer tailing.
  1.    The first step is the RNA: DNA hybrid synthesis
  2.    Terminal transferase is used for the addition of nucleotides on 3’ end
  3.    Terminal transferase adds only at DNA strands
  4.    The DNA strand is now having known sequence at 3’ end
 Discuss Question
Answer: Option C. -> Terminal transferase adds only at DNA strands
Answer: (c).Terminal transferase adds only at DNA strands
Question 74. The first primer in the case of 3’ RACE is ___________
  1.    internal sequence
  2.    oligo-dT adaptor molecule
  3.    oligo-dA adaptor molecule
  4.    adaptor oligo-dT primer
 Discuss Question
Answer: Option B. -> oligo-dT adaptor molecule
Answer: (b).oligo-dT adaptor molecule
Question 75. The first cDNA strand in 5’ RACE is tailed with oligo-dA tail.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 76. Which of the following is desirable?
  1.    Driver- driver hybrid
  2.    Driver- tracer hybrid
  3.    Tracer- tracer hybrid
  4.    Driver alone
 Discuss Question
Answer: Option C. -> Tracer- tracer hybrid
Answer: (c).Tracer- tracer hybrid
Question 77. If physical methods are used, tracer is amplified and is labelled with biotin.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option B. -> False
Answer: (b).False
Question 78. The process of finding a particular member of the library which is having some defined properties is called as ___________
  1.    searching
  2.    screening
  3.    locating
  4.    narrowing
 Discuss Question
Answer: Option B. -> screening
Answer: (b).screening
Question 79. Which of the statement holds true?
  1.    The tracer is treated with an enzyme which generates compatible ends
  2.    The driver is treated with an enzyme which generates compatible ends
  3.    Both driver and tracer are treated with an enzyme to generate compatible ends
  4.    Both driver and tracer are treated with an enzyme to generate incompatible ends
 Discuss Question
Answer: Option A. -> The tracer is treated with an enzyme which generates compatible ends
Answer: (a).The tracer is treated with an enzyme which generates compatible ends
Question 80. If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________
  1.    annotation
  2.    database search
  3.    in silico
  4.    electronic search
 Discuss Question
Answer: Option C. -> in silico
Answer: (c).in silico

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