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MCQs

Total Questions : 113 | Page 4 of 12 pages
Question 31. Complete digestion is preferred over partial digestion for the fragmentation of DNA.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option B. -> False
Answer: (b).False
Question 32. To avoid ligation of separate DNA fragments, which of the enzyme is used?
  1.    phosphatase
  2.    kinase
  3.    ligase
  4.    endonuclease
 Discuss Question
Answer: Option A. -> phosphatase
Answer: (a).phosphatase
Question 33. Vector and insert are mixed, ligated and packaged and introduced into the host by ___________
  1.    transformation
  2.    transduction
  3.    infection
  4.    transformation and infection
 Discuss Question
Answer: Option D. -> transformation and infection
Answer: (d).transformation and infection
Question 34. Libraries constructed in plasmid vectors can be kept as ___________
  1.    plasmid containing cells
  2.    naked DNA
  3.    both plasmid containing cells and naked DNA
  4.    naked DNA is preferred over plasmid containing cells
 Discuss Question
Answer: Option C. -> both plasmid containing cells and naked DNA
Answer: (c).both plasmid containing cells and naked DNA
Question 35. Libraries using phage cloning vectors are often kept as ___________
  1.    unpackaged phage
  2.    packaged phage
  3.    both packaged and unpackaged phage
  4.    both packaged and unpackaged phage are used but packaged is favoured
 Discuss Question
Answer: Option B. -> packaged phage
Answer: (b).packaged phage
Question 36. Choose the incorrect statement for cDNA libraries.
  1.    They constitute of DNA copies produced from the RNA sequences and usually mRNA
  2.    They represent expressed sequences
  3.    Introns are not represented
  4.    Comparison of cDNA sequences with genomic sequences leads to the determination of polyadenylation sites
 Discuss Question
Answer: Option C. -> Introns are not represented
Answer: (c).Introns are not represented
Question 37. A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________
  1.    expressed RNA sequences
  2.    expressed sequence tags (ESTs)
  3.    expressed cDNA sequences
  4.    library
 Discuss Question
Answer: Option B. -> expressed sequence tags (ESTs)
Answer: (b).expressed sequence tags (ESTs)
Question 38. Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?
  1.    Polyadenylation should be at 3’ end
  2.    Eukaryotic mRNAs are mostly non-polyadenylated
  3.    Bacterial mRNAs and organelle mRNAs are polyadenylated
  4.    It is carried out by the addition of T residues after synthesis
 Discuss Question
Answer: Option A. -> Polyadenylation should be at 3’ end
Answer: (a).Polyadenylation should be at 3’ end
Question 39. During storage, there are chances of degradation of DNA. Larger fragments are having more chances of being got lost.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 40. Choose the incorrect statement for oligo-dT cellulose.
  1.    It is used for separation of polyadenylated mRNA from another mRNA
  2.    oligo-dT are covalently attached to the solid support via OH bonds
  3.    A solution containing RNA is passed through the column
  4.    Poly A tail attaches to the oligo-dT by ionic bonds
 Discuss Question
Answer: Option D. -> Poly A tail attaches to the oligo-dT by ionic bonds
Answer: (d).Poly A tail attaches to the oligo-dT by ionic bonds

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