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MCQs

Total Questions : 113 | Page 3 of 12 pages
Question 21. In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 22. In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.
  1.    DNA binding, activation
  2.    Activation, DNA binding
  3.    Activation, transcription
  4.    DNA binding, transcription
 Discuss Question
Answer: Option A. -> DNA binding, activation
Answer: (a).DNA binding, activation
Question 23. For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.
  1.    prey
  2.    bait
  3.    binding
  4.    activation
 Discuss Question
Answer: Option B. -> bait
Answer: (b).bait
Question 24. Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?
  1.    Bacterial host
  2.    Fungal host
  3.    Parasitic host
  4.    Yeast host
 Discuss Question
Answer: Option D. -> Yeast host
Answer: (d).Yeast host
Question 25. Sequences that can function as origins of replication are called as ___________
  1.    partial replicating sequences
  2.    self replicating sequences
  3.    autonomously replicating sequences
  4.    modified replicating sequences
 Discuss Question
Answer: Option C. -> autonomously replicating sequences
Answer: (c).autonomously replicating sequences
Question 26. The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?
  1.    deactivated
  2.    activated
  3.    destroyed
  4.    may be activated or not
 Discuss Question
Answer: Option D. -> may be activated or not
Answer: (d).may be activated or not
Question 27. Protein-protein interactions such as in electron transport lead to activation of the reporter gene.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option B. -> False
Answer: (b).False
Question 28. In humans, diploid cells contain
  1.    23 chromosomes
  2.    46 chromosomes
  3.    47 chromosomes
  4.    24 chromosomes
 Discuss Question
Answer: Option B. -> 46 chromosomes
Answer: (b).46 chromosomes
Question 29. Independent assortment of genes occurs during meiosis in
  1.    prokaryotes
  2.    non-living organisms
  3.    eukaryotes
  4.    unicellular colonies
 Discuss Question
Answer: Option C. -> eukaryotes
Answer: (c).eukaryotes
Question 30. _____ should enter the cell in the case of activation of the reporter gene for two hybrid system.
  1.    Bait protein
  2.    Prey protein
  3.    Both bait and prey protein
  4.    Either one of them
 Discuss Question
Answer: Option C. -> Both bait and prey protein
Answer: (c).Both bait and prey protein

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