Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 10 of 12 pages

Question 91. If the screening of the DNA constructs is carried out in pooled batches then it takes less time.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 92. Introns are ________ in nature and the sequence is _______ than exons.

coding, less random
non-coding, less random
non-coding, more random
coding, more random

Discuss Question

Answer: Option C. -> non-coding, more random
Answer: (c).non-coding, more random

Question 93. If the genome size is small, then sequencing of the entire genome is ___________

easy
difficult
independent of the size of genome
not possible

Discuss Question

Answer: Option A. -> easy
Answer: (a).easy

Question 94. Choose the incorrect statement for the open reading frame.

It is helpful in deciding whether the clone is correct or not
It consists of an initiation codon
It consists of a termination codon
The presence of introns doesn’t affect the open reading frame

Discuss Question

Answer: Option D. -> The presence of introns doesn’t affect the open reading frame
Answer: (d).The presence of introns doesn’t affect the open reading frame

Question 95. Choose the correct statement with respect to the bacterial genome.

They are easy to sequence
They are difficult to sequence
Methods such as transcription and translation are satisfactory
Only transcription can be carried out

Discuss Question

Answer: Option B. -> They are difficult to sequence
Answer: (b).They are difficult to sequence

Question 96. For identification of genes that are expressed under special conditions, _______ libraries are used.

cDNA
genomic
subtractive
shelf

Discuss Question

Answer: Option C. -> subtractive
Answer: (c).subtractive

Question 97. GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

It stands for Green Fluorescent Protein
It is obtained from a bio-luminescent jellyfish and produces protein aequorin which emits blue light
The blue light is produced because of binding of sodium ions
The absorbed blue light produces green light which can be detected further

Discuss Question

Answer: Option C. -> The blue light is produced because of binding of sodium ions
Answer: (c).The blue light is produced because of binding of sodium ions

Question 98. Luciferase genes are also used at times for detection. Choose the correct statement for them.

They are obtained from fire flies only
The detection requires provision of substrate which produces light
Enzymes such as beta-galactosidase requires substrate X-gluc to produce light
Lucifearse genes are preferred over fluorescent proteins

Discuss Question

Answer: Option B. -> The detection requires provision of substrate which produces light
Answer: (b).The detection requires provision of substrate which produces light

Question 99. Promoter-probe vectors are used often. Choose the correct statement for these vectors.

They are used for identifying sequences that can function as promoter in vivo
It contains a reporter gene which contains its promoter along
The reporter gene is having a cloning site
After insertion of DNA into the cloning site, selection of plasmids is carried out by blue white screening

Discuss Question

Answer: Option A. -> They are used for identifying sequences that can function as promoter in vivo
Answer: (a).They are used for identifying sequences that can function as promoter in vivo

Question 100. In ______ organisms, firstly the gene is looked into model organisms.