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Question
During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it?
Options:
A .  Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus
B .  Direct sequencing can be used if the template DNA is heterozygous at the locus
C .  If cloning is done before sequencing, then it is detected via using only a single clone for sequencing
D .  In the case several recombinants are used, it can’t go undetected
Answer: Option B
Answer: (b).Direct sequencing can be used if the template DNA is heterozygous at the locus

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