Questions and answers for exam Preparation

MCQs

Total Questions : 113 | Page 1 of 12 pages

Question 1. What is the second primer in the case of 5’ RACE?

Internal primer
Oligo-dA sequence
Adaptor-oligo-dT primer
Oligo-dT adaptor molecule

Discuss Question

Answer: Option C. -> Adaptor-oligo-dT primer
Answer: (c).Adaptor-oligo-dT primer

Question 2. Choose the correct statement for modification of homopolymer tailing method.

It includes modification of primers
Primers are varied by simply altering their size by randomly adding or removing bases
The 5’ end of the first cDNA strand is tailed with C residues
A single stranded oilgonucleotide is then used for second strand synthesis

Discuss Question

Answer: Option A. -> It includes modification of primers
Answer: (a).It includes modification of primers

Question 3. By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 4. RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

Blunt ended ligation
Addition of linkers
Using appropriate restriction enzymes
All the methods can be used equivalently

Discuss Question

Answer: Option D. -> All the methods can be used equivalently
Answer: (d).All the methods can be used equivalently

Question 5. Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________

amplification
normalization
selection
narrowing

Discuss Question

Answer: Option B. -> normalization
Answer: (b).normalization

Question 6. Choose the correct statement in order to enrich rare species.

The most basic principle relies on the kinetics of hybridization
A collection of single stranded molecules is allowed to reanneal under given conditions
The strands which are less abundant are able to find their complement easily
The left over molecules are more abundant in nature

Discuss Question

Answer: Option A. -> The most basic principle relies on the kinetics of hybridization
Answer: (a).The most basic principle relies on the kinetics of hybridization

Question 7. Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having.

The driver and tracer are mixed in double stranded form
Tracer is in stoichiometric excess in comparison to driver
Tracer and driver are in the same amount
The sequences are allowed to hybridize

Discuss Question

Answer: Option D. -> The sequences are allowed to hybridize
Answer: (d).The sequences are allowed to hybridize

Question 8. Which statement holds true for hydroxyapatite?

It binds to single stranded molecules more tightly than double stranded molecules
It binds to linear molecules more tightly than circular molecules
It binds to circular molecules more tightly than linear molecules
It binds to double stranded molecules more tightly than single stranded molecules

Discuss Question

Answer: Option D. -> It binds to double stranded molecules more tightly than single stranded molecules
Answer: (d).It binds to double stranded molecules more tightly than single stranded molecules

Question 9. Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________