12th Grade > Biology
BIOTECHNOLOGY PRINCIPLES AND PROCESSES MCQs
Total Questions : 42
| Page 3 of 5 pages
Answer: Option A. -> 1970
:
A
Traditional biotechnology dates back to the ancient civilization. However, modern biotechnology which involves the use of genetic engineering and R-DNA technology was developed in the 1970s.
:
A
Traditional biotechnology dates back to the ancient civilization. However, modern biotechnology which involves the use of genetic engineering and R-DNA technology was developed in the 1970s.
Answer: Option B. -> Plasmids
:
B
Plasmids are used as vectors to transfer gene of interest into the recipient organism. Plasmids are found natually in some bacteria, however, artifically built plasmids such as pBR 322 are also available.
:
B
Plasmids are used as vectors to transfer gene of interest into the recipient organism. Plasmids are found natually in some bacteria, however, artifically built plasmids such as pBR 322 are also available.
Answer: Option C. -> Arber, Nathans & Smith
:
C
Arber was the first to discover the Type I restriction enzyme. In 1970Hamilton Smith discovered the type II restriction enzyme. Later Daniel Nathan showed that these enzymes could be used to map the DNA. For their effort, they were awarded the Nobel Prize in 1978.
:
C
Arber was the first to discover the Type I restriction enzyme. In 1970Hamilton Smith discovered the type II restriction enzyme. Later Daniel Nathan showed that these enzymes could be used to map the DNA. For their effort, they were awarded the Nobel Prize in 1978.
Answer: Option A. -> An Escherichia coli plasmid was used to transfer penicillin resistance from S. aureus to E.coli
:
A
Cohen and his coworkers for the first time introduced genes belonging to a different species in bacterium E.coli. They used penicillin resistance gene from a plasmid in another bacterium S.aureus. This plasmid was cut using EcoRI.The plasmid had four restriction sites and generated four fragments one of which contained gene of interest , penicillin resistance gene.The E.coli plasmid pSC101 was used as vector which had tetracycline resistance gene. The recombinant E. Coli had dual resistance over tetracycline as well as penicillin.
:
A
Cohen and his coworkers for the first time introduced genes belonging to a different species in bacterium E.coli. They used penicillin resistance gene from a plasmid in another bacterium S.aureus. This plasmid was cut using EcoRI.The plasmid had four restriction sites and generated four fragments one of which contained gene of interest , penicillin resistance gene.The E.coli plasmid pSC101 was used as vector which had tetracycline resistance gene. The recombinant E. Coli had dual resistance over tetracycline as well as penicillin.
Answer: Option B. -> Aerobic and anaerobic Bioreactors
:
B
Based on the type of organism, the reactors are classified as aerobic and anaerobic bioreactors. This classification is done due to the fact that certain organisms can survive without oxygen and some require oxygen to grow. Therefore, based on this, some bioreactors will have provisions for an inlet of oxygen and some will not.
:
B
Based on the type of organism, the reactors are classified as aerobic and anaerobic bioreactors. This classification is done due to the fact that certain organisms can survive without oxygen and some require oxygen to grow. Therefore, based on this, some bioreactors will have provisions for an inlet of oxygen and some will not.
Answer: Option B. -> Amplify genes
:
B
PCR is a method used in R DNA technology work to amplifly the amount of DNA molecule. When a gene of interest is extracted, the quantity is very low. Using PCR, the quantity of the gene can be amplified by over 10,000X times.
:
B
PCR is a method used in R DNA technology work to amplifly the amount of DNA molecule. When a gene of interest is extracted, the quantity is very low. Using PCR, the quantity of the gene can be amplified by over 10,000X times.
Answer: Option A. -> Make cuts at specific positions within the DNA molecule
:
A
Restriction endonucleases recognises a specific DNA base sequence (recognition sequence, recognizion site, restriction sequence or restriction site having palindromic sequence) and cleaves both the strands of DNA at or near the site. The enzyme cuts the DNA, generating restriction fragments with overhanging ends (sticky ends) or blunt ends.
:
A
Restriction endonucleases recognises a specific DNA base sequence (recognition sequence, recognizion site, restriction sequence or restriction site having palindromic sequence) and cleaves both the strands of DNA at or near the site. The enzyme cuts the DNA, generating restriction fragments with overhanging ends (sticky ends) or blunt ends.
Answer: Option A. -> [A] is true and [R] is false.
:
A
Process of clumping together of cells using inorganic salts or mineral acids is called as flocculation. For recovering products present in the media, cells need to be separated from the media. To facilitate separation using centrifugation, cells are put through process of flocculation in which organic salts or mineral acids are used to make cells clump together. This increases their density as compared to the surrounding media helping in easy separation.
:
A
Process of clumping together of cells using inorganic salts or mineral acids is called as flocculation. For recovering products present in the media, cells need to be separated from the media. To facilitate separation using centrifugation, cells are put through process of flocculation in which organic salts or mineral acids are used to make cells clump together. This increases their density as compared to the surrounding media helping in easy separation.
Answer: Option A. -> Filtration
:
A
Filtration is the process used for separating suspended particles like cell debris and suspended solids from the fermentation mix. The process is similar to straining tea using a tea strainer. As the liquid is passed through a porous medium, liquids and gases pass through while solids are retained.
:
A
Filtration is the process used for separating suspended particles like cell debris and suspended solids from the fermentation mix. The process is similar to straining tea using a tea strainer. As the liquid is passed through a porous medium, liquids and gases pass through while solids are retained.
Answer: Option D. -> Ti Plasmid
:
D
Ti Plasmids are obtained from the organism Agrobacterium tumefaciens. This plasmid natually induces tumor in plants. The plasmid is modified such that the tumor inducing gene is removed and the gene of interest is added. This plasmid is the introduced into the host plant cells and the gene is added into the plant genome.
:
D
Ti Plasmids are obtained from the organism Agrobacterium tumefaciens. This plasmid natually induces tumor in plants. The plasmid is modified such that the tumor inducing gene is removed and the gene of interest is added. This plasmid is the introduced into the host plant cells and the gene is added into the plant genome.
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