12th Grade > Biology
BIOTECHNOLOGY PRINCIPLES AND PROCESSES MCQs
Total Questions : 42
| Page 4 of 5 pages
Answer: Option C. -> Both [A] and [R] are true and [R] is a correct explanation to [A].
:
C
Floatation is a technique used for cell separation. In this process air bubbles are pumped through the fermentation mix. This creates foam and along with it cells also come to the surface and float. These floating cells are easy to separate. This process is easy to perform as it does not involve any addition of chemicals.
:
C
Floatation is a technique used for cell separation. In this process air bubbles are pumped through the fermentation mix. This creates foam and along with it cells also come to the surface and float. These floating cells are easy to separate. This process is easy to perform as it does not involve any addition of chemicals.
Answer: Option B. -> E. coli
:
B
The ori site of pBR322 has been take from ColE1 plasmid. Due to this, the ori site of pBR322 is compatible with the E.coli system. This also helps the plasmid to maintain a high copy number inside the cell.
:
B
The ori site of pBR322 has been take from ColE1 plasmid. Due to this, the ori site of pBR322 is compatible with the E.coli system. This also helps the plasmid to maintain a high copy number inside the cell.
Question 33. Some of the steps involved in the production of human insulin are given below. Choose the correct sequence
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli
i. Obtaining of gene for human insulin using c-DNA technology
ii. Culturing recombinant E-coli in bioreactors
iii. Purification of human insulin
iv. Insertion of human insulin gene into plasmid
v. Introduction of recombinant plasmid into E.coli
vi. Extraction of recombinant gene product from E-coli
Answer: Option C. -> i-iv-v-ii-vi-iii
:
C
First the human insulin gene is obtained using c-DNA technology and then inserted into a plasmid. The recmbinant plasmid is introduced to E.coli by transformation.The transformed E.coli is selectively cultured inbioreactors and the Insulin protein in extracted and subsequently purified in what is known as downstream processing.
:
C
First the human insulin gene is obtained using c-DNA technology and then inserted into a plasmid. The recmbinant plasmid is introduced to E.coli by transformation.The transformed E.coli is selectively cultured inbioreactors and the Insulin protein in extracted and subsequently purified in what is known as downstream processing.
Answer: Option A. -> True
:
A
Ampicillin resistance gene is the most commonly used selection marker. When the plasmid containing this marker is transformed, it gives cells the ability to thrive in media containing ampicillin. The non-transformed cells die due to the presence of antibiotic.
:
A
Ampicillin resistance gene is the most commonly used selection marker. When the plasmid containing this marker is transformed, it gives cells the ability to thrive in media containing ampicillin. The non-transformed cells die due to the presence of antibiotic.
Answer: Option A. -> 1000 litres
:
A
Bioreactors are used to produce a large number of the transgenic organism. The size and capacity of bioreactors can be different. However, 1000 litres is usually the maximum capacity of a bioreactor used in any biotechnology industry. Beyond that, it can be slightly complicated to control the growth and extraction of product.
:
A
Bioreactors are used to produce a large number of the transgenic organism. The size and capacity of bioreactors can be different. However, 1000 litres is usually the maximum capacity of a bioreactor used in any biotechnology industry. Beyond that, it can be slightly complicated to control the growth and extraction of product.
Answer: Option B. -> Restriction enzyme used has recognition sites in the gene
:
B
When you choose a restriction enzyme for isolating the gene of interest, you need to ensure that the enzyme should not have a restriction site in the middle of the gene. This way, the whole gene can be obtained and later used in further processes. In this case, the gene probably had a restriction site within its sequence for the restriction enzyme used. This resulted in fragmentation of the gene of interest.
:
B
When you choose a restriction enzyme for isolating the gene of interest, you need to ensure that the enzyme should not have a restriction site in the middle of the gene. This way, the whole gene can be obtained and later used in further processes. In this case, the gene probably had a restriction site within its sequence for the restriction enzyme used. This resulted in fragmentation of the gene of interest.
Answer: Option B. -> Thermus aquaticus
:
B
Thermus aquaticus is an archaebacteria that can survive in high temperatures. Due to this, their enzymes are capable of working in unusually high temperature and perfectly suitable for PCR since it employs temperatures as high as 96∘C. At this high temperature, most enzymes occurring in nature get denatured. Taq polymerase however has heat resistant capabilities.
:
B
Thermus aquaticus is an archaebacteria that can survive in high temperatures. Due to this, their enzymes are capable of working in unusually high temperature and perfectly suitable for PCR since it employs temperatures as high as 96∘C. At this high temperature, most enzymes occurring in nature get denatured. Taq polymerase however has heat resistant capabilities.
Answer: Option A. -> Hydrogen bonds
:
A
Hydrogen bonds are present in between complementary bases. This complementary base pairing holds the two antiparallel strands of DNA together. There are 3 hydrogen bonds between G and C while 2 are present in between A and T.
:
A
Hydrogen bonds are present in between complementary bases. This complementary base pairing holds the two antiparallel strands of DNA together. There are 3 hydrogen bonds between G and C while 2 are present in between A and T.
Answer: Option C. -> Clone is a result of sexual reproduction
:
C
A clone consists of theasexual progeny of a single individual or cell. The process of producing an identicalcopy of selfis known as cloning. All the individuals of a clone have the same genotype, which is also identical with that of the 'parent' individual from which the clone was taken. Since a clone is not derived from sexual reproduction, no interchange of gametes takes place and hence no interchange of genetic information happens therefore further bringing importance to the fact that clones are genetically identical to each other and the 'parent' clone.
:
C
A clone consists of theasexual progeny of a single individual or cell. The process of producing an identicalcopy of selfis known as cloning. All the individuals of a clone have the same genotype, which is also identical with that of the 'parent' individual from which the clone was taken. Since a clone is not derived from sexual reproduction, no interchange of gametes takes place and hence no interchange of genetic information happens therefore further bringing importance to the fact that clones are genetically identical to each other and the 'parent' clone.
Answer: Option A. -> True
:
A
Downstream processing involves separation of product and purification of the product. Separating the product from reaction mix itself brings about partial purification as it is separated from other impurities and therefore it can be considered as the first step in product purification.
:
A
Downstream processing involves separation of product and purification of the product. Separating the product from reaction mix itself brings about partial purification as it is separated from other impurities and therefore it can be considered as the first step in product purification.
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