12th Grade > Biology
BIOTECHNOLOGY PRINCIPLES AND PROCESSES MCQs
Total Questions : 42
| Page 5 of 5 pages
Answer: Option B. -> False
:
B
Replicon consists of origin of replication site and other controlling genes that helps in the replication of the plasmid. For a plasmid to be viable vector, it needs to be self replicating. The replicon in the plasmid is responsible its replication. To select for transformed organisms, usually a gene carrying antibitoic resistance like ampicillin or kanamycin are inserted into the plasmid vector and the organisms are grown in media containing antibitoic so that in theory, only those with the gene for antibiotic resistance will survive and hence can be selected.
:
B
Replicon consists of origin of replication site and other controlling genes that helps in the replication of the plasmid. For a plasmid to be viable vector, it needs to be self replicating. The replicon in the plasmid is responsible its replication. To select for transformed organisms, usually a gene carrying antibitoic resistance like ampicillin or kanamycin are inserted into the plasmid vector and the organisms are grown in media containing antibitoic so that in theory, only those with the gene for antibiotic resistance will survive and hence can be selected.
Question 42. Regarding assertion and reason, select the correct option
Assertion: Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.
Reason: A gene incorporated in the bacterial chromosomal gene gets automatically transferred to the crop with which the bacterium is associated.
Assertion: Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.
Reason: A gene incorporated in the bacterial chromosomal gene gets automatically transferred to the crop with which the bacterium is associated.
Answer: Option D. -> Both the assertion and reason are false.
:
D
Agrobacterium tumefaciensinfects certain plants, in which Ti plasmid (not chromosomal genome) causes the formation of tumour like growth called a crown gall. To insert a gene into the plant, the tumor inducing gene is removed from the Ti plasmid and the gene of interest is inserted into the plasmid. The bacteria is then allowed to infect the plant. The plamsid is trasnfered into the plant genome as it happens in the natural Ti plasmid and the gene of interest gets incorporated into the genome of the plant.
:
D
Agrobacterium tumefaciensinfects certain plants, in which Ti plasmid (not chromosomal genome) causes the formation of tumour like growth called a crown gall. To insert a gene into the plant, the tumor inducing gene is removed from the Ti plasmid and the gene of interest is inserted into the plasmid. The bacteria is then allowed to infect the plant. The plamsid is trasnfered into the plant genome as it happens in the natural Ti plasmid and the gene of interest gets incorporated into the genome of the plant.
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