Question
Cohen and his coworkers transferred genes from S. aureus to E.coli. Which of the following statement is correct regarding this experiment?
Answer: Option A
:
A
Cohen and his coworkers for the first time introduced genes belonging to a different species in bacterium E.coli. They used penicillin resistance gene from a plasmid in another bacterium S.aureus. This plasmid was cut using EcoRI.The plasmid had four restriction sites and generated four fragments one of which contained gene of interest , penicillin resistance gene.The E.coli plasmid pSC101 was used as vector which had tetracycline resistance gene. The recombinant E. Coli had dual resistance over tetracycline as well as penicillin.
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:
A
Cohen and his coworkers for the first time introduced genes belonging to a different species in bacterium E.coli. They used penicillin resistance gene from a plasmid in another bacterium S.aureus. This plasmid was cut using EcoRI.The plasmid had four restriction sites and generated four fragments one of which contained gene of interest , penicillin resistance gene.The E.coli plasmid pSC101 was used as vector which had tetracycline resistance gene. The recombinant E. Coli had dual resistance over tetracycline as well as penicillin.
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