Questions and answers for exam Preparation

MCQs

Total Questions : 89 | Page 9 of 9 pages

Question 81. Circularization must take place in the bacterial cells before the replication starts.

True
False
May be True or False
Can't Say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 82. How many origins of replication are there once the cyclization is carried out by loxP sites?

Discuss Question

Answer: Option B. -> 2
Answer: (b).2

Question 83. Choose the correct statement in respect to sacB gene?

The cloning site for PAC lies on the side of sacB gene
It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose
Expression of sacB in the presence of sucrose is beneficial
Levan is non-toxic for E. coli

Discuss Question

Answer: Option B. -> It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose
Answer: (b).It is responsible for producing an enzyme which is responsible for catalysing sucrose into glucose and fructose

Question 84. Choose the incorrect statement for PAC vectors.

In these vectors, the phage packaging signals are removed but the two P1 origin of replication persist
They resemble BAC vectors
They accept an insert of upto 300 kbp
Instability is observed

Discuss Question

Answer: Option D. -> Instability is observed
Answer: (d).Instability is observed

Question 85. The immediate early transcripts direct synthesis of which genes?

N genes
cro genes
Both N and cro genes
PR and PL genes

Discuss Question

Answer: Option C. -> Both N and cro genes
Answer: (c).Both N and cro genes

Question 86. What is the function of the N gene?

It promotes the function of terminators
It overrides the function of terminators
It promotes the production of the cro gene
It is responsible for making the infectious particle linear

Discuss Question

Answer: Option B. -> It overrides the function of terminators
Answer: (b).It overrides the function of terminators

Question 87. Choose the correct statement for cII gene and its related function?

The transcription is extended to the region of cII gene and it is termed as late transcription
They are responsible for deactivating the promoter for repressor establishment
This promoter is responsible to decide whether the life cycle would be lysogenic or lytic
cII gene is responsible for other gene which is gene cIII

Discuss Question

Answer: Option C. -> This promoter is responsible to decide whether the life cycle would be lysogenic or lytic
Answer: (c).This promoter is responsible to decide whether the life cycle would be lysogenic or lytic

Question 88. Choose the incorrect statement for lysogenic mode.

PRE forms cI gene product which is responsible for activation of promoters
The cII protein is responsible for interfering with cro and Q proteins and they are required for lytic life cycle
The cII protein activates the promoter PINT
The Int protein with host protein is responsible for site specific recombination

Discuss Question

Answer: Option A. -> PRE forms cI gene product which is responsible for activation of promoters
Answer: (a).PRE forms cI gene product which is responsible for activation of promoters

Question 89. Which gene is required for the lysogenic phage to be adopted?