Questions and answers for exam Preparation

MCQs

Total Questions : 89 | Page 5 of 9 pages

Question 41. Choose incorrect statement for the substitution vector EMBL4.

It is having two single multiple cloning sites
The region to be removed is on one side of the multiple cloning sites
If a digestion within the multiple cloning sites, it generates central portion and right and left arms
The right and left portions are called as arms

Discuss Question

Answer: Option B. -> The region to be removed is on one side of the multiple cloning sites
Answer: (b).The region to be removed is on one side of the multiple cloning sites

Question 42. Is it necessary to stop the relegation of the central portion before the stiffer fragment is ligated.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 43. What happens if chain-termination mutation is in the S gene?

Cell lysis is blocked
Growth of cells containing low levels of packaging proteins is not allowed
The lysis of cells is not carried artificially
Packaging is not carried out efficiently

Discuss Question

Answer: Option A. -> Cell lysis is blocked
Answer: (a).Cell lysis is blocked

Question 44. Which of the following is used for blocking the phage-encoded recombination?

Mutation in D gene
Mutation in E gene
Red mutation
Mutation in S gene

Discuss Question

Answer: Option C. -> Red mutation
Answer: (c).Red mutation

Question 45. Which of the following is not a consequence of deletion in the b region?

Excision of the prophage on induction is prevented
It reduces the amount of endogenous phage DNA in the packaging mix
It reduces the amount of exogenous phage DNA in the packaging mix
It reduces the level of background non-recombinant phage

Discuss Question

Answer: Option C. -> It reduces the amount of exogenous phage DNA in the packaging mix
Answer: (c).It reduces the amount of exogenous phage DNA in the packaging mix

Question 46. Once cosmids are inside the E.coli cells, they don’t generate phage but are propagated as plasmids.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 47. Which size of the insert is accepted by the cosmids?

10-20 kbp
35-45 kbp
50-60 kbp
100-120 kbp

Discuss Question

Answer: Option B. -> 35-45 kbp
Answer: (b).35-45 kbp

Question 48. Which of the following is the correct method to check whether the DNA has entered into the cell or not in the case of cosmid?

If a transformation has taken place turbid plaques are formed
If a transformation has taken place clear plaques are formed
If a transformation has taken place, it can be confirmed via ampicillin resistance
If a transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also

Discuss Question

Answer: Option C. -> If a transformation has taken place, it can be confirmed via ampicillin resistance
Answer: (c).If a transformation has taken place, it can be confirmed via ampicillin resistance

Question 49. What happens once the cosmid enters the E. coli cells?