Questions and answers for exam Preparation

MCQs

Total Questions : 89 | Page 8 of 9 pages

Question 71. If a single system is used for packaging, there are increased chances of endogenous material being packed.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 72. What is the function of red and gam gene products?

It promotes the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P1
It inhibits the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P2
It inhibits the growth of the phage in the E. coli cells which are lytic for bacteriophage P1
It activates the growth of the phage in the E. coli cells which are lytic for bacteriophage P2

Discuss Question

Answer: Option B. -> It inhibits the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P2
Answer: (b).It inhibits the growth of the phage in the E. coli cells which are lysogenic for bacteriophage P2

Question 73. Phages which are designated as spi-are __________

red+ gam+
red+ gam-
red- gam+
red- gam-

Discuss Question

Answer: Option D. -> red- gam-
Answer: (d).red- gam-

Question 74. The red and gam genes are removed in which type of phages?

Substitution phage
Replacement and substitution phages both
Replacement phage
Substitution is preferred over replacement phage

Discuss Question

Answer: Option C. -> Replacement phage
Answer: (c).Replacement phage

Question 75. The cI function can be scanned in order to check whether the recombinants are present or not. Which of the following doesn’t hold true?

The cI protein is required for the formation of lysogen
The plaques formed are turbid in the case if the cI gene is active
The plaques formed are clear in the case if the cI gene is inactive
hflA mutant host reduces the amount of cII stability

Discuss Question

Answer: Option D. -> hflA mutant host reduces the amount of cII stability
Answer: (d).hflA mutant host reduces the amount of cII stability

Question 76. Bacteriophage Mu is also a phage, choose the correct statement for it.

It is an example of temperate phage
It is packaged into heads containing 50 kbp of DNA
When the phage replicates it goes transposition only a few times
Mu can’t be used for cloning in vivo

Discuss Question

Answer: Option A. -> It is an example of temperate phage
Answer: (a).It is an example of temperate phage

Question 77. Which of the following is not included as a step for cloning mini Mu in vivo?

A culture of target strain, whose DNA we want to clone is infected with mini Mu
The phage is inserted at multiple sites in the genome
Some of the packaged strain is flanked by two mini Mu genes
The packaged phage is introduced into a second strain and Mu would replicate as a phage

Discuss Question

Answer: Option D. -> The packaged phage is introduced into a second strain and Mu would replicate as a phage
Answer: (d).The packaged phage is introduced into a second strain and Mu would replicate as a phage

Question 78. Choose the incorrect statement for ‘Mini Mu’?