Questions and answers for exam Preparation

MCQs

Total Questions : 89 | Page 7 of 9 pages

Question 61. Gene V blocks the production of which gene?

Gene III
Gene II
Gene VIII
Gene IV

Discuss Question

Answer: Option B. -> Gene II
Answer: (b).Gene II

Question 62. What is the size of the DNA molecule which can be packed by the viral protein coat?

100kbp
200kbp
500kbp
No size restriction

Discuss Question

Answer: Option D. -> No size restriction
Answer: (d).No size restriction

Question 63. The uniform layer of cells is known as lawn and the holes peppered from infected cells are termed as __________

plaques
holes
colony
pothole

Discuss Question

Answer: Option A. -> plaques
Answer: (a).plaques

Question 64. M13 doesn’t actually lyse the cells.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 65. The plaques formed by M13 infection are called as __________

retarded plaques
true plaques
pseudoplaques
lysogenized plaques

Discuss Question

Answer: Option C. -> pseudoplaques
Answer: (c).pseudoplaques

Question 66. M13 vector is tightly packed. Where is the available site to insert DNA?

In the gene II
Between gene II and IV
Between gene III and V
Between gene V and VIII

Discuss Question

Answer: Option B. -> Between gene II and IV
Answer: (b).Between gene II and IV

Question 67. Choose the correct statement for the synthesis of RNA probe?

Double stranded DNA is only used for probe synthesis
Single stranded DNA can be used for probe synthesis
Probes are prepared for RNA transcripts which are specific for only particular strand
Probes are prepared for RNA transcripts which are specific for both the strands

Discuss Question

Answer: Option B. -> Single stranded DNA can be used for probe synthesis
Answer: (b).Single stranded DNA can be used for probe synthesis

Question 68. Choose the incorrect statement with respect to the collection of single stranded molecules.

An infected culture is set up and after a while the cells are removed by centrifugation
The phage is in the supernatant and is precipitated by addition of glycol and sodium chloride
The phage pellet is resuspended and phenol is used for removal of protein
The single stranded molecules sediment at the bottom

Discuss Question

Answer: Option D. -> The single stranded molecules sediment at the bottom
Answer: (d).The single stranded molecules sediment at the bottom

Question 69. Which of the statement doesn’t holds for phage display systems?

The filamentous phage is often used as phage display system
Coding sequences are inserted in one of the protein coating genes
The most common protein coding genes are gene II or gene VIII
The inserted is expressed along with other protein coat genes

Discuss Question

Answer: Option C. -> The most common protein coding genes are gene II or gene VIII
Answer: (c).The most common protein coding genes are gene II or gene VIII

Question 70. Choose the correct statement for Sanger’s method of sequencing.