Questions and answers for exam Preparation

MCQs

Total Questions : 89 | Page 1 of 9 pages

Question 1. At times, a second enzyme is used while the central portion is removed and the stuffer fragment is placed there. Choose the statement which doesn’t holds for this process.

It is a method which is used for avoiding the relegation of central portion before the stuffer fragment is attached over there
In this method, the central portion is cut with another enzyme after being separated from the arms
The arms are also cut with this second enzyme
It is not necessary to remove the fragments generated

Discuss Question

Answer: Option C. -> The arms are also cut with this second enzyme
Answer: (c).The arms are also cut with this second enzyme

Question 2. Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________

A gene
D gene
E and D gene
D and A gene

Discuss Question

Answer: Option C. -> E and D gene
Answer: (c).E and D gene

Question 3. Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.

Separate strains with chain-termination mutations in different genes for coat components are used
Temperature sensitive cI repressor is used
Due to heat shock packaging proteins are induced
Packaging is carried out in each strain

Discuss Question

Answer: Option D. -> Packaging is carried out in each strain
Answer: (d).Packaging is carried out in each strain

Question 4. Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it.

Packaging in phage coat is a more efficient process than the normal transformation process
The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration
Lysate constitutes of lambda proteins needed for phage assembly
The amount of non-recombinant phage in the background is less

Discuss Question

Answer: Option D. -> The amount of non-recombinant phage in the background is less
Answer: (d).The amount of non-recombinant phage in the background is less

Question 5. Physical separation is also used at times. Choose the correct statement with respect to this method.

Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient
In sugar density gradient, the molecules are separated on the basis of density
The central portion is recovered from the gel or the gradient
Gel is more efficient than the sugar density gradient

Discuss Question

Answer: Option A. -> Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient
Answer: (a).Physical separation constitutes of gel electrophoresis and or by using centrifugation in sugar density gradient

Question 6. The essential sites for recombination are known as __________

chi sites
rec sites
gam sites
red sites

Discuss Question

Answer: Option A. -> chi sites
Answer: (a).chi sites

Question 7. Choose the correct statement for RecBCD nuclease.

It promotes rolling circle replication
It is blocked by Gam protein
It blocks theta mode of replication
It is blocked by Red protein

Discuss Question

Answer: Option B. -> It is blocked by Gam protein
Answer: (b).It is blocked by Gam protein

Question 8. There is a limit on upper size of the DNA to be packed. Choose the correct statement with respect to phages in this context.

There is some phage DNA lost in this process
The phages are known as transformed phages
These type of phages can’t be selected and harvested
Lambda is not a special attachment site

Discuss Question

Answer: Option A. -> There is some phage DNA lost in this process
Answer: (a).There is some phage DNA lost in this process

Question 9. The replication rate remains the same for all the phages irrespective of what sequence is there in the phage.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 10. There are some phages which don’t preferentially transduce some special regions of the phage genome. These phages are known as __________