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MCQs

Total Questions : 71 | Page 6 of 8 pages
Question 51. The change in nucleotide sequence of a short region of genome is called
  1.    replication
  2.    recombination
  3.    repair
  4.    mutation
 Discuss Question
Answer: Option D. -> mutation
Answer: (d).mutation
Question 52. If a functional gene is disrupted while disrupting a restriction site _______ is created.
  1.    frameshift mutation
  2.    point mutation
  3.    either of the mutations
  4.    any other kind of mutation
 Discuss Question
Answer: Option A. -> frameshift mutation
Answer: (a).frameshift mutation
Question 53. When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:
  1.    linker scanning
  2.    generation of deletions
  3.    mutation
  4.    replacement
 Discuss Question
Answer: Option A. -> linker scanning
Answer: (a).linker scanning
Question 54. Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.
  1.    not only similar, also exactly same
  2.    similar, not same
  3.    not similar, is entirely different
  4.    different, near
 Discuss Question
Answer: Option B. -> similar, not same
Answer: (b).similar, not same
Question 55. Additional restriction sites can be introduced near the existing restriction site by mutagenesis.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 56. Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.
  1.    double stranded ended
  2.    circular molecules
  3.    single stranded ends
  4.    supercoiled molecules
 Discuss Question
Answer: Option C. -> single stranded ends
Answer: (c).single stranded ends
Question 57. Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?
  1.    1
  2.    2
  3.    3
  4.    4
 Discuss Question
Answer: Option B. -> 2
Answer: (b).2
Question 58. It is easier to subclone a restriction fragment if it belongs to?
  1.    small gene
  2.    large gene
  3.    prokaryotic organism
  4.    eukaryotic organism
 Discuss Question
Answer: Option B. -> large gene
Answer: (b).large gene
Question 59. How many sites can be mutated at a time?
  1.    1
  2.    2
  3.    3
  4.    Many
 Discuss Question
Answer: Option D. -> Many
Answer: (d).Many
Question 60. Several different mutations can be induced at one site.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True

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