Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease. Options: A . &nbspdouble stranded ended B . &nbspcircular molecules C . &nbspsingle stranded ends D . &nbspsupercoiled molecules

Small deletions at a restriction site can be generated by cutting and degrading

Question

Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

Options:

A . double stranded ended

B . circular molecules

C . single stranded ends

D . supercoiled molecules

Answer: Option C
Answer: (c).single stranded ends

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Question 1. Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecu....coli for replication, how many types of molecules are produced?

Question 2. It is easier to subclone a restriction fragment if it belongs to?....

Question 3. How many sites can be mutated at a time?....

Question 4. Several different mutations can be induced at one site.....

Question 5. If mixed oligonucleotides are used, it is regarded as ______________....

Question 6. What is the function of ung gene?....

Question 7. It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of t....in finding out _________ role of the wild type gene.

Question 8. The minor change in amino acid sequence can lead to _______ effect on three dimensional structure and there _____ in the....three dimensional structure and there _____ in the primary sequence.

Question 9. DpnI cuts ________ strands.....

Question 10. The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence ....in the absence of background expression of wild type gene.

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