Questions and answers for exam Preparation

MCQs

Total Questions : 71 | Page 4 of 8 pages

Question 31. Choose the incorrect statement for the method based on antisense RNA.

RNA is synthesized complementary to the sequence of the gene which is to be inactivated
It is achieved by placing a DNA sequence which encodes RNA complementary to the RNA to be inactivated
Expression of the endogenous gene is diminished
This method is preferred over gene disruption has gene inactivation can be achieved completely

Discuss Question

Answer: Option D. -> This method is preferred over gene disruption has gene inactivation can be achieved completely
Answer: (d).This method is preferred over gene disruption has gene inactivation can be achieved completely

Question 32. Direct alteration of particular parts of protein as a way of probing the relationship between structure and function is termed as:

genetic engineering
protein engineering
alteration of protein function
structure engineering

Discuss Question

Answer: Option B. -> protein engineering
Answer: (b).protein engineering

Question 33. Approaches for creating mutations can be divided into how many types?

Discuss Question

Answer: Option B. -> 2
Answer: (b).2

Question 34. At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

reverse genetics
protein engineering
mutation
location of function

Discuss Question

Answer: Option A. -> reverse genetics
Answer: (a).reverse genetics

Question 35. In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 36. If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

complete
partial
carried out by a different enzyme then that the restriction site
carried out by a mixture of restriction enzymes

Discuss Question

Answer: Option B. -> partial
Answer: (b).partial

Question 37. The ________ protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP.

cre recombinase
gene II
gene IV
gene VIII

Discuss Question

Answer: Option A. -> cre recombinase
Answer: (a).cre recombinase

Question 38. SiRNAs can either be introduced directly or by microinjection.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 39. Introduction into host organism can also be done by using a DNA construct, which when transcribed, generates an RNA which is _________

circular
linear
double stranded
self-complementary

Discuss Question

Answer: Option D. -> self-complementary
Answer: (d).self-complementary

Question 40. Choose the incorrect statement for cre-lox excision.

The chromosomal copy of the target gene replaces the target gene flanked by loxP sites
The second step is supply of Cre recombinase
Integration of cre takes place under a controllable promoter followed by induction of the promoter
Induction results in expression of cre, recombination along loxP sites and excision of the sequence between

Discuss Question

Answer: Option A. -> The chromosomal copy of the target gene replaces the target gene flanked by loxP sites
Answer: (a).The chromosomal copy of the target gene replaces the target gene flanked by loxP sites