Questions and answers for exam Preparation

MCQs

Total Questions : 71 | Page 2 of 8 pages

Question 11. Transition occurs when purine is substituted with

pyrimidine
pyrol
purine
Amino acids

Discuss Question

Answer: Option C. -> purine
Answer: (c).purine

Question 12. Bisulphite ions are used to deaminate ________ residues in _______ DNA.

C, double stranded
C, single stranded
U, double stranded
U, single stranded

Discuss Question

Answer: Option B. -> C, single stranded
Answer: (b).C, single stranded

Question 13. For mutagenesis without PCR, which of the following can be used as a template?

Single stranded DNA
Double stranded DNA
Circular DNA
Both single and double stranded DNA

Discuss Question

Answer: Option D. -> Both single and double stranded DNA
Answer: (d).Both single and double stranded DNA

Question 14. An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

complementary
non-complementary
can either be complementary or non-complementary
not related

Discuss Question

Answer: Option A. -> complementary
Answer: (a).complementary

Question 15. If the template is double stranded, they need to be separated before annealing of oligonucleotide.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 16. Which of the following statement is incorrect for the synthesis of the second strand?

The oligonucleotide is acting as a primer for the synthesis of the second strand
DNA polymerase and dNTPs are added for synthesis
The polymerase should have 5’-3’ exonuclease activity
A polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence

Discuss Question

Answer: Option C. -> The polymerase should have 5’-3’ exonuclease activity
Answer: (c).The polymerase should have 5’-3’ exonuclease activity

Question 17. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

two, two
two, three
one, two
one, three

Discuss Question

Answer: Option B. -> two, three
Answer: (b).two, three

Question 18. Choose the correct statement.

There are two flanking primers and they are having mutations
There is one primer which anneals the target sequence and is having mutation
Either of the flanking primers or the primer is annealing to the target sequence is having mutation
One of the flanking primer and the primer annealing to the target sequence is having mutation

Discuss Question

Answer: Option B. -> There is one primer which anneals the target sequence and is having mutation
Answer: (b).There is one primer which anneals the target sequence and is having mutation

Question 19. Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.