Questions and answers for exam Preparation

MCQs

Total Questions : 71 | Page 7 of 8 pages

Question 61. If mixed oligonucleotides are used, it is regarded as ______________

mixed mutagenesis
multiple mutagenesis
cassette mutagenesis
polymutagenesis

Discuss Question

Answer: Option C. -> cassette mutagenesis
Answer: (c).cassette mutagenesis

Question 62. What is the function of ung gene?

It is responsible for deamination of cytosine
It is responsible for deamination of uracil
It is responsible for removal of uracil
It is responsible for removal of cytosine

Discuss Question

Answer: Option C. -> It is responsible for removal of uracil
Answer: (c).It is responsible for removal of uracil

Question 63. It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of the wild type gene.

biological
chemical
physiological
anatomical

Discuss Question

Answer: Option C. -> physiological
Answer: (c).physiological

Question 64. The minor change in amino acid sequence can lead to _______ effect on three dimensional structure and there _____ in the primary sequence.

huge, may be no change
no, may be huge change
very less, is very less change
huge, would be huge change

Discuss Question

Answer: Option A. -> huge, may be no change
Answer: (a).huge, may be no change

Question 65. DpnI cuts ________ strands.

methylated
non-methylated
phosphorylated
non-phosphorylated

Discuss Question

Answer: Option A. -> methylated
Answer: (a).methylated

Question 66. The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence of background expression of wild type gene.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 67. How can mutant strains be produced?

In systematic mutagenesis programmes
In individual organisms
Both by individual organisms and systematic mutagenesis programmes
Apart from these two, other methods are also used

Discuss Question

Answer: Option C. -> Both by individual organisms and systematic mutagenesis programmes
Answer: (c).Both by individual organisms and systematic mutagenesis programmes

Question 68. The gene to be disrupted is cloned and a selectable marker is inserted. What should be the effect of selectable marker?

It should have no effect on target gene
It should make the target gene non-functional
There is no restriction; it can be either functional or non-functional
It should improvise the chances of survival of the target gene

Discuss Question

Answer: Option B. -> It should make the target gene non-functional
Answer: (b).It should make the target gene non-functional

Question 69. Stable acquisition of the marker can take place only if a double crossover over the flanking sequence and their chromosomal counterparts causes the marker’s integration into the chromosome.