Questions and answers for exam Preparation

MCQs

Total Questions : 71 | Page 3 of 8 pages

Question 21. PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 22. For the selection of the molecules having mutated sequence, which of the statement is true?

It is suitable for methods which are PCR based
It is suitable for methods which are not PCR based
It is suitable for both PCR and not PCR based
Selection of molecules with the mutant sequence is not possible

Discuss Question

Answer: Option B. -> It is suitable for methods which are not PCR based
Answer: (b).It is suitable for methods which are not PCR based

Question 23. Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

mutS mutation
mutD mutation
mutE mutation
mutG mutation

Discuss Question

Answer: Option A. -> mutS mutation
Answer: (a).mutS mutation

Question 24. Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.

A vector is used which is having antibiotic resistance gene
Apart from antibiotic resistance gene, a second antibiotic resistance gene is also present
There are two mutagenic primers which are used
The second strand synthesis is carried out by only one primer

Discuss Question

Answer: Option D. -> The second strand synthesis is carried out by only one primer
Answer: (d).The second strand synthesis is carried out by only one primer

Question 25. Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 26. In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

Magnesium
Calcium
Sodium
Sulphur

Discuss Question

Answer: Option D. -> Sulphur
Answer: (d).Sulphur

Question 27. If ______ gene is there, the double crossover may leave ________ in the chromosome.

linear, functional copy
circular, functional copy
linear, a non-functional copy
circular, a non-functional copy

Discuss Question

Answer: Option B. -> circular, functional copy
Answer: (b).circular, functional copy

Question 28. If the target organism contains more than one copy of the gene, what is the effect on these copies?

Only copy is disrupted
All the copies are disrupted
It is difficult to ensure that all the copies are disrupted
Only a specified number of copies are disrupted

Discuss Question

Answer: Option C. -> It is difficult to ensure that all the copies are disrupted
Answer: (c).It is difficult to ensure that all the copies are disrupted

Question 29. If methods are based on cellular processes that lead to inactivation of gene expression by affecting the RNA, then it is called as ___________