Which of the following statement is incorrect for the synthesis of the second strand? Options: A . &nbspThe oligonucleotide is acting as a primer for the synthesis of the second strand B . &nbspDNA polymerase and dNTPs are added for synthesis C . &nbspThe polymerase should have 5’-3’ exonuclease activity D . &nbspA polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence

Which of the following statement is incorrect for the synthesis of the second st

Question

Which of the following statement is incorrect for the synthesis of the second strand?

Options:

A . The oligonucleotide is acting as a primer for the synthesis of the second strand

B . DNA polymerase and dNTPs are added for synthesis

C . The polymerase should have 5’-3’ exonuclease activity

D . A polymerase having 5’-3’ exonuclease activity would degrade the primer that carries the mutant sequence

Answer: Option C
Answer: (c).The polymerase should have 5’-3’ exonuclease activity

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Question 1. A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.....

Question 2. Choose the correct statement.....

Question 3. Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.....

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Question 5. PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a produc....the part of the gene. It is called as megaprimer.

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