Questions and answers for exam Preparation

MCQs

Total Questions : 120 | Page 10 of 12 pages

Question 91. Which of the following is incorrect regarding 2D-Page?

It stands for Two-dimensional polyacrylamide gel electrophoresis
It separates proteins by charge only
The gel is run in one direction in a pH gradient under a non-denaturing condition
It works to separate proteins by isoelectric points (pI)

Discuss Question

Answer: Option B. -> It separates proteins by charge only
Answer: (b).It separates proteins by charge only

Question 92. The classic protein separation methods involve two-dimensional gel electrophoresis followed by gel image analysis.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 93. Which of the following is incorrect regarding Mass Spectrometry Protein Identification?

The proteolysis doesn’t generate a pattern according to molecular weight
Proteins can be identified and characterized using MS
The proteins from a two dimensional gel system are first digested in situ with a protease
Protein spots of interest are excised from the two-dimensional gel

Discuss Question

Answer: Option A. -> The proteolysis doesn’t generate a pattern according to molecular weight
Answer: (a).The proteolysis doesn’t generate a pattern according to molecular weight

Question 94. Which of the following is incorrect regarding 2D-Page?

Not all proteins can be separated by this method or stained properly
The stained gel can be scanned and digitized for image analysis
Membrane proteins are largely hydrophilic and readily solubilized
One of the challenges of this technique is the separation of membrane proteins

Discuss Question

Answer: Option C. -> Membrane proteins are largely hydrophilic and readily solubilized
Answer: (c).Membrane proteins are largely hydrophilic and readily solubilized

Question 95. Comparing two-dimensional gel images from various experiments can sometimes pose a challenge because the gels, unlike DNA microarrays, may shrink or warp.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 96. The signal sequences are typically _______ residues long, rich in _____ charged residues such as arginines as well as hydroxyl residues such as serines and threonines, but devoid of ______ charged residues.

28 to 80, positively, negatively
300 to 800, negatively, positively
28 to 80, negatively, positively
300 to 500, positively, negatively

Discuss Question

Answer: Option A. -> 28 to 80, positively, negatively
Answer: (a).28 to 80, positively, negatively

Question 97. The signal sequences have a _________ consensus but contain some specific features. They all have a ______ core region preceded by one or more positively charged residues.

weak, hydrophilic
weak, hydrophobic
strong, hydrophilic
strong, hydrophilic

Discuss Question

Answer: Option B. -> weak, hydrophobic
Answer: (b).weak, hydrophobic

Question 98. For many eukaryotic proteins, newly synthesized protein precursors have to be transported to specific membrane-bound compartments and be proteolytically processed to become functional.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 99. Once the proteins are translocated within the organelles, protease cleavage takes place to remove the signal sequences and generate mature proteins.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 100. Which of the following is an incorrect statement about the terminologies related to protein sorting?

Subcellular localization is an integral part of protein functionality
Many proteins exhibit functions only after being transported to certain compartments of the cell
All the proteins exhibit functions after being transported to certain compartments of the cell
Protein sorting is also known as protein targeting

Discuss Question

Answer: Option C. -> All the proteins exhibit functions after being transported to certain compartments of the cell
Answer: (c).All the proteins exhibit functions after being transported to certain compartments of the cell