Questions and answers for exam Preparation

MCQs

Total Questions : 120 | Page 2 of 12 pages

Question 11. Which of the following is untrue about SAGE?

This approach is much more efficient than the EST analysis
This approach is quite less efficient than the EST analysis
It uses a short nucleotide tag to define a gene transcript
It allows sequencing of multiple tags in a single clone

Discuss Question

Answer: Option B. -> This approach is quite less efficient than the EST analysis
Answer: (b).This approach is quite less efficient than the EST analysis

Question 12. Which of the following is untrue about SAGE?

Sequencing is the most costly and time-consuming step
Here, sequencing is economical but time-consuming step
Sequencing is economical but time-reducing step
It is difficult to know how many tags need to be sequenced to get a good coverage of the entire transcriptome

Discuss Question

Answer: Option A. -> Sequencing is the most costly and time-consuming step
Answer: (a).Sequencing is the most costly and time-consuming step

Question 13. Which of the following is untrue about the drawbacks of SAGE?

One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification
Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all
Correctly sequenced SAGE tag always corresponds to several genes
The drawback with this approach is the sensitivity to sequencing errors

Discuss Question

Answer: Option C. -> Correctly sequenced SAGE tag always corresponds to several genes
Answer: (c).Correctly sequenced SAGE tag always corresponds to several genes

Question 14. SAGEmap is a SAGE database created by NCBI.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option A. -> True
Answer: (a).True

Question 15. SAGExProfiler doesn’t provide information about overexpressed or silenced genes.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 16. Which of the following is untrue about SAGE Genie?

It is an NCBI web-based program
It allows matching of experimentally obtained SAGE tags to known genes
It provides an interface for visualizing human gene expression
It doesn’t filter out linker sequences from experimentally obtained SAGE tags

Discuss Question

Answer: Option D. -> It doesn’t filter out linker sequences from experimentally obtained SAGE tags
Answer: (d).It doesn’t filter out linker sequences from experimentally obtained SAGE tags

Question 17. Which of the following is an incorrect statement?

Collecting a SAGE library is very labor intensive and expensive
Collecting a SAGE library is quite economical
SAGE is not suitable for rapid screening of cells
Gene identification from SAGE data is also more cumbersome

Discuss Question

Answer: Option B. -> Collecting a SAGE library is quite economical
Answer: (b).Collecting a SAGE library is quite economical

Question 18. DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.

True
False
May be True or False
Can't say

Discuss Question

Answer: Option B. -> False
Answer: (b).False

Question 19. Which of the following is an incorrect statement?

SAGE and DNA microarrays are both high throughput techniques that determine global mRNA expression levels
Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other
SAGE does not require prior knowledge of the transcript sequence
DNA microarray experiments can only detect the genes spotted on the microarray

Discuss Question

Answer: Option B. -> Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other
Answer: (b).Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other

Question 20. The PCR amplification step involved in the SAGE procedure means that it requires a large quantity of sample mRNA.