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MCQs

Total Questions : 222 | Page 22 of 23 pages
Question 211. . The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
  1.    cos site
  2.    flanking sequences
  3.    border sequences
  4.    transfer sequences
 Discuss Question
Answer: Option C. -> border sequences
Question 212. . T-DNA transfer and processing into plant genome requires products of which of the following genes?
  1.    vir A,B
  2.    vir G,C
  3.    vir D,E
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 213. . The transfer of intermediate vectors into Agrobacterium are brought about by
  1.    transformation
  2.    biparental mating
  3.    triparental mating
  4.    transduction
 Discuss Question
Answer: Option C. -> triparental mating
Question 214. . Microinjection involves
  1.    injection of large amount of DNA
  2.    injection of DNA into bigger cells
  3.    injection with needle having diameter greater than cell diameter
  4.    all of the above
 Discuss Question
Answer: Option C. -> injection with needle having diameter greater than cell diameter
Question 215. . In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
  1.    vir A
  2.    vir B
  3.    vir C
  4.    vir D
 Discuss Question
Answer: Option D. -> vir D
Question 216. . Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
  1.    pBR 322 based plasmid vector
  2.    pRK 2013
  3.    pRN 3
  4.    all of these
 Discuss Question
Answer: Option A. -> pBR 322 based plasmid vector
Question 217. . Which of the following are used as selection marker for the cells transformed with Agrobacterium?
  1.    Neomycin phosphotransferase
  2.    Streptomycin phosphotransferase
  3.    Hygromycin phosphotransferase
  4.    any of the above
 Discuss Question
Answer: Option D. -> any of the above
Question 218. . Vir genes required for the T-DNA transfer and processing are located
  1.    on the T-DNA
  2.    outside the T-DNA region
  3.    on the plant genome
  4.    none of these
 Discuss Question
Answer: Option B. -> outside the T-DNA region
Question 219. . 
Plant transformation vectors based on Agrobacterium can generally be divided into
  1.    two vectors
  2.    four vectors
  3.    six vectors
  4.    eight vectors
 Discuss Question
Answer: Option A. -> two vectors
Question 220. . Which of the following are used as selection marker for the cells transformed with Agrobacterium?
  1.    Neomycin phosphotransferase
  2.    Streptomycin phosphotransferase
  3.    Hygromycin phosphotransferase
  4.    any of the above
 Discuss Question
Answer: Option D. -> any of the above

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