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MCQs

Total Questions : 222 | Page 18 of 23 pages
Question 171. Opine synthesis is the property
  1.    determined by the bacteria Agrobacterium tumefaciens
  2.    conferred to plant cells when it transformed by Agrobacterium tumefaciens
  3.    both (a) and (b)
  4.    of normal plant cells
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 172. The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
  1.    transfer sequences
  2.    border sequences
  3.    flanking sequences
  4.    cos site
 Discuss Question
Answer: Option B. -> border sequences
Question 173. Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
  1.    four sets of sequence necessary for gene transfer
  2.    three sets of sequence necessary for gene transfer
  3.    one set of sequence necessary for gene transfer
  4.    two sets of sequence necessary for gene transfer
 Discuss Question
Answer: Option D. -> two sets of sequence necessary for gene transfer
Question 174. In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
  1.    vir D
  2.    vir C
  3.    vir A
  4.    vir B
 Discuss Question
Answer: Option A. -> vir D
Question 175. Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
  1.    pRN 3
  2.    all of these
  3.    pRK 2013
  4.    pBR 322 based plasmid vector
 Discuss Question
Answer: Option D. -> pBR 322 based plasmid vector
Question 176. T-DNA transfer and processing into plant genome requires products of which of the following genes?
  1.    vir D,E
  2.    vir G,C
  3.    vir A,B
  4.    All of these
 Discuss Question
Answer: Option D. -> All of these
Question 177. Microinjection involves
  1.    All of these
  2.    injection of DNA into bigger cells
  3.    injection with needle having diameter greater than cell diameter
  4.    injection of large amount of DNA
 Discuss Question
Answer: Option C. -> injection with needle having diameter greater than cell diameter
Question 178. The transfer of intermediate vectors into Agrobacterium are brought about by
  1.    transformation
  2.    biparental mating
  3.    transduction
  4.    triparental mating
 Discuss Question
Answer: Option D. -> triparental mating
Question 179. Plant transformation vectors based on Agrobacterium can generally be divided into
  1.    two vectors
  2.    six vectors
  3.    eight vectors
  4.    four vectors
 Discuss Question
Answer: Option A. -> two vectors
Question 180. Vir genes required for the T-DNA transfer and processing are located
  1.    on the plant genome
  2.    on the T-DNA
  3.    none of these
  4.    outside the T-DNA region
 Discuss Question
Answer: Option D. -> outside the T-DNA region

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