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MCQs

Total Questions : 222 | Page 19 of 23 pages
Question 181. Which of the following are used as selection marker for the cells transformed with Agrobacterium?
  1.    Hygromycin phosphotransferase
  2.    any of the above
  3.    Streptomycin phosphotransferase
  4.    Neomycin phosphotransferase
 Discuss Question
Answer: Option B. -> any of the above
Question 182.  Virulence trait of Agrobacterium tumefaciens is borne on
  1.    chromosomal DNA
  2.    tumour inducing plasmid DNA
  3.    both chromosomal and plasmid DNA
  4.    cryptic plasmid DNA
 Discuss Question
Answer: Option B. -> tumour inducing plasmid DNA
Question 183.  Which of the following is not true about the helper plasmids?
  1.    These can replicate in Agrobacterium
  2.    These help in the mediating conjugation of intermediate vectors
  3.    These can't replicate in Agrobacterium
  4.    All of the above
 Discuss Question
Answer: Option A. -> These can replicate in Agrobacterium
Question 184.  The size of the virulent plasmid of Agrobacterium tumefaciens is
  1.    40-80 kb
  2.    80-120 kb
  3.    140-235 kb
  4.    >235 kb
 Discuss Question
Answer: Option C. -> 140-235 kb
Question 185.  Co-integrating transformation vectors must include a region of homology in
  1.    the vector plasmid
  2.    the Ti-plasmid
  3.    between vector plasmid and Ti-plasmid
  4.    none of these
 Discuss Question
Answer: Option C. -> between vector plasmid and Ti-plasmid
Question 186.  In a plant tumour cell
  1.    complete Ti plasmid is incorporated in plant nuclear DNA
  2.    different parts of the Ti-plasmid are incorporated
  3.    only a small specific segment of callus T DNA is incorporated
  4.    may vary from plant to plant
 Discuss Question
Answer: Option C. -> only a small specific segment of callus T DNA is incorporated
Question 187.  Which technique is used to introduce genes into dicots?
  1.    electroporation
  2.    particle acceleration
  3.    microinjection
  4.    Ti plasmid infection
 Discuss Question
Answer: Option D. -> Ti plasmid infection
Question 188.  Direct DNA uptake by protoplasts can be stimulated by
  1.    polyethylene glycol (PEG)
  2.    decanal
  3.    luciferin
  4.    all of these
 Discuss Question
Answer: Option A. -> polyethylene glycol (PEG)
Question 189.  Crown gall tissue
  1.    can be cultivated in vitro in absence of bacteria
  2.    retains tumorous properties when cultivated
  3.    both (a) and (b)
  4.    shows tumorous properties only in presence of bacteria
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 190. . Opines are
  1.    amino acid derivatives found in tumor tissues
  2.    amino acid derivatives found in normal tissues
  3.    amino acid derivatives found in both normal as well as tumor tissues
  4.    none of the above
 Discuss Question
Answer: Option A. -> amino acid derivatives found in tumor tissues

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