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MCQs

Total Questions : 222 | Page 21 of 23 pages
Question 201. . Liposomes mediated gene transfer in plants involves
  1.    plasmid DNA enclosed in a lipid bag
  2.    fusion of liposomes with protoplast
  3.    use of polyethylene glycol (PEG)
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
Question 202. . Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
  1.    Acetosyringone
  2.    α-hydroxy syringone
  3.    Both (a) and (b)
  4.    None of these
 Discuss Question
Answer: Option C. -> Both (a) and (b)
Question 203. . 
Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
  1.    pRK 2013
  2.    pRN 3
  3.    either (a) or (b)
  4.    none of these
 Discuss Question
Answer: Option C. -> either (a) or (b)
Question 204. . Which of the following is not true about the direct repeats flanking T-DNA?
  1.    They are conserved between nopaline and octopine Ti-plasmids
  2.    These repeats are transferred intact to the plant genome
  3.    These are important in integration mechanism
  4.    all of the above
 Discuss Question
Answer: Option B. -> These repeats are transferred intact to the plant genome
Question 205. . The right segment of octopine T-DNA (TR) is necessary for
  1.    enzymes for agropine biosynthesis
  2.    tumour formation
  3.    conjugative transfer
  4.    all of these
 Discuss Question
Answer: Option A. -> enzymes for agropine biosynthesis
Question 206. . Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?
  1.    It is carried out with the help of micromanipulator
  2.    The recipient cells are immobilized on artificial support or artificially bound to substarate
  3.    It employs needle with diameter greater than cell diameter
  4.    all of the above
 Discuss Question
Answer: Option C. -> It employs needle with diameter greater than cell diameter
Question 207. . Which of the following is true about T DNA?
  1.    Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
  2.    Integration of T DNA occurs only at one specific sites in the plant nuclear DNA
  3.    Integration of T DNA occurs at two specific sites in the plant nuclear DNA
  4.    Integration of T DNA occurs at one site that may be random in the plant nuclear DNA
 Discuss Question
Answer: Option A. -> Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA
Question 208. . The left segment of octopine T-DNA (TL) is necessary for
  1.    enzymes for agropine biosynthesis
  2.    tumour formation
  3.    conjugative transfer
  4.    all of these
 Discuss Question
Answer: Option B. -> tumour formation
Question 209. . Opine synthesis is the property
  1.    conferred to plant cells when it transformed by Agrobacterium tumefaciens
  2.    determined by the bacteria Agrobacterium tumefaciens
  3.    both (a) and (b)
  4.    of normal plant cells
 Discuss Question
Answer: Option C. -> both (a) and (b)
Question 210. . 
Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain
  1.    one set of sequence necessary for gene transfer
  2.    two sets of sequence necessary for gene transfer
  3.    three sets of sequence necessary for gene transfer
  4.    four sets of sequence necessary for gene transfer
 Discuss Question
Answer: Option B. -> two sets of sequence necessary for gene transfer

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