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MCQs

Total Questions : 96 | Page 9 of 10 pages
Question 81. . Single stranded vectors are useful
  1.    for sequencing of cloned DNA
  2.    for oligonucleotide directed mutagenesis
  3.    for probe preparation
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
Question 82. . Inserted DNA in λ gt 11 can be expressed as
  1.    β-galactosidase fused protein
  2.    free protein in the cytoplasm
  3.    free protein that is secreted out
  4.    all of the above
 Discuss Question
Answer: Option A. -> β-galactosidase fused protein
Question 83. . EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
  1.    6-7 kb
  2.    15-25 kb
  3.    40-44 kb
  4.    1-2 kb
 Discuss Question
Answer: Option B. -> 15-25 kb
Question 84. . Size of the DNA that can be packaged into a λ phage is
  1.    50 kb
  2.    35-53 kb
  3.    40-50 kb
  4.    any size
 Discuss Question
Answer: Option B. -> 35-53 kb
Question 85. . 
Plasmids which are maintained as limited number of copies per cell are known as
  1.    stringent plasmids
  2.    relaxed plasmids
  3.    cryptic plasmids
  4.    all of these
 Discuss Question
Answer: Option A. -> stringent plasmids
Question 86. . Phagemid consist of
  1.    plasmid vector carrying λ phage's cos site
  2.    plasmid vector carrying λ attachment (λ att) site
  3.    plasmid vector carrying origin of replication of λ phage only
  4.    plasmid vector carrying origin of replication of plasmid only
 Discuss Question
Answer: Option B. -> plasmid vector carrying λ attachment (λ att) site
Question 87. . Charon 34 and Charon 35 are the examples of
  1.    plasmid vector
  2.    cosmid vector
  3.    phage vector
  4.    phagemid vector
 Discuss Question
Answer: Option C. -> phage vector
Question 88. . Cosmid vectors are used for
  1.    cloning small fragments of DNA
  2.    cloning large fragments of DNA
  3.    cloning prokaryotic DNA only
  4.    cloning eukaryotic DNA only
 Discuss Question
Answer: Option B. -> cloning large fragments of DNA
Question 89. . Cryptic plasmids
  1.    do not exhibit any phenotypic trait
  2.    exhibit many phenotypic traits
  3.    exhibit one phenotypic traits
  4.    exhibit antibiotic resistance
 Discuss Question
Answer: Option A. -> do not exhibit any phenotypic trait
Question 90. . Maximum size of foreign DNA that can be inserted into an insertion vector is
  1.    35 kb
  2.    18 kb
  3.    50 kb
  4.    27 kb
 Discuss Question
Answer: Option B. -> 18 kb

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