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MCQs

Total Questions : 96 | Page 3 of 10 pages
Question 21. Conjugative plasmids
  1.    exhibit antibiotic resistance
  2.    do not exhibit antibiotic resistance
  3.    carry transfer genes called the tra genes
  4.    do not carry transfer genes
 Discuss Question
Answer: Option C. -> carry transfer genes called the tra genes
Answer: (c).carry transfer genes called the tra genes
Question 22. Maximum size of foreign DNA that can be inserted into a replacement vector is
  1.    25-30 kb
  2.    18-20 kb
  3.    20-25 kb
  4.    40-50 kb
 Discuss Question
Answer: Option C. -> 20-25 kb
Answer: (c).20-25 kb
Question 23. Which of the following is not true about phagemid?
  1.    Contain functional origin of replication of the plasmid and λ phage
  2.    May be propagated as a plasmid or as phage in appropriate strain
  3.    Contain λ att site
  4.    Can only be propagated as phage
 Discuss Question
Answer: Option D. -> Can only be propagated as phage
Answer: (d).Can only be propagated as phage
Question 24. A plasmid can be considered as a suitable cloning vector if
  1.    it can be readily isolated from the cells
  2.    it possesses a single restriction site for one or more restriction enzymes
  3.    insertion of foreign DNA does not alter its replication properties
  4.    All of the above
 Discuss Question
Answer: Option D. -> All of the above
Answer: (d).All of the above
Question 25. PBR 322 has/have which of the following selection marker(s)?
  1.    Ampr
  2.    Tetr
  3.    Both (a) and (b)
  4.    Kanr
 Discuss Question
Answer: Option C. -> Both (a) and (b)
Answer: (c).Both (a) and (b)
Question 26. Difference between λ gt 10 and λ gt 11 vectors is that
  1.    λ gt 11 is an expression vector
  2.    λ gt 10 is an expression vector
  3.    λ gt 10 is a replacement vector
  4.    λ gt 11 is a replacement vector
 Discuss Question
Answer: Option A. -> λ gt 11 is an expression vector
Answer: (a).λ gt 11 is an expression vector
Question 27. Plasmids which are maintained as multiple copy number per cell are known as
  1.    stringent plasmids
  2.    relaxed plasmids
  3.    cryptic plasmids
  4.    none of these
 Discuss Question
Answer: Option B. -> relaxed plasmids
Answer: (b).relaxed plasmids
Question 28. Maximum size of foreign DNA that can be inserted into an insertion vector is
  1.    35 kb
  2.    18 kb
  3.    50 kb
  4.    27 kb
 Discuss Question
Answer: Option B. -> 18 kb
Answer: (b).18 kb
Question 29. Phagemid consist of
  1.    plasmid vector carrying λ phage's cos site
  2.    plasmid vector carrying λ attachment (λ att) site
  3.    plasmid vector carrying origin of replication of λ phage only
  4.    plasmid vector carrying origin of replication of plasmid only
 Discuss Question
Answer: Option B. -> plasmid vector carrying λ attachment (λ att) site
Answer: (b).plasmid vector carrying λ attachment (λ att) site
Question 30. Cryptic plasmids
  1.    do not exhibit any phenotypic trait
  2.    exhibit many phenotypic traits
  3.    exhibit one phenotypic traits
  4.    exhibit antibiotic resistance
 Discuss Question
Answer: Option A. -> do not exhibit any phenotypic trait
Answer: (a).do not exhibit any phenotypic trait

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