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MCQs

Total Questions : 96 | Page 6 of 10 pages
Question 51. Inserted DNA in λ gt 11 can be expressed as
  1.    All of these
  2.    free protein in the cytoplasm
  3.    β-galactosidase fused protein
  4.    free protein that is secreted out
 Discuss Question
Answer: Option C. -> β-galactosidase fused protein
Question 52. EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
  1.    6-7 kb
  2.    40-44 kb
  3.    15-25 kb
  4.    1-2 kb
 Discuss Question
Answer: Option C. -> 15-25 kb
Question 53. Size of the DNA that can be packaged into a λ phage is
  1.    40-50 kb
  2.    any size
  3.    35-53 kb
  4.    50 kb
 Discuss Question
Answer: Option C. -> 35-53 kb
Question 54. Cosmid vectors are used for
  1.    cloning large fragments of DNA
  2.    cloning eukaryotic DNA only
  3.    cloning prokaryotic DNA only
  4.    cloning small fragments of DNA
 Discuss Question
Answer: Option A. -> cloning large fragments of DNA
Question 55. Plasmids which are maintained as limited number of copies per cell are known as
  1.    all of these
  2.    stringent plasmids
  3.    relaxed plasmids
  4.    cryptic plasmids
 Discuss Question
Answer: Option B. -> stringent plasmids
Question 56. Charon 34 and Charon 35 are the examples of
  1.    phagemid vector
  2.    plasmid vector
  3.    cosmid vector
  4.    phage vector
 Discuss Question
Answer: Option D. -> phage vector
Question 57. Maximum size of foreign DNA that can be inserted into an insertion vector is
  1.    50 kb
  2.    35 kb
  3.    18 kb
  4.    27 kb
 Discuss Question
Answer: Option C. -> 18 kb
Question 58. Cryptic plasmids
  1.    do not exhibit any phenotypic trait
  2.    exhibit antibiotic resistance
  3.    exhibit one phenotypic traits
  4.    exhibit many phenotypic traits
 Discuss Question
Answer: Option A. -> do not exhibit any phenotypic trait
Question 59. Phagemid consist of
  1.    plasmid vector carrying origin of replication of plasmid only
  2.    plasmid vector carrying origin of replication of λ phage only
  3.    plasmid vector carrying λ phage's cos site
  4.    plasmid vector carrying λ attachment (λ att) site
 Discuss Question
Answer: Option D. -> plasmid vector carrying λ attachment (λ att) site
Question 60. Plasmids which are maintained as multiple copy number per cell are known as
  1.    relaxed plasmids
  2.    cryptic plasmids
  3.    stringent plasmids
  4.    none of these
 Discuss Question
Answer: Option A. -> relaxed plasmids

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