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MCQs

Total Questions : 96 | Page 2 of 10 pages
Question 11. Plasmids which are maintained as limited number of copies per cell are known as
  1.    stringent plasmids
  2.    relaxed plasmids
  3.    cryptic plasmids
  4.    all of these
 Discuss Question
Answer: Option A. -> stringent plasmids
Answer: (a).stringent plasmids
Question 12. Cos site of the cosmids
  1.    consists of 12 bases
  2.    helps whole genome in circularization and ligation
  3.    both (a) and (b)
  4.    contains cleavage site
 Discuss Question
Answer: Option C. -> both (a) and (b)
Answer: (c).both (a) and (b)
Question 13. Single stranded vectors are useful
  1.    for sequencing of cloned DNA
  2.    for oligonucleotide directed mutagenesis
  3.    for probe preparation
  4.    all of the above
 Discuss Question
Answer: Option D. -> all of the above
Answer: (d).all of the above
Question 14. Phagemid vectors are
  1.    combination of plasmid and phage λ
  2.    combination of phages and cosmid
  3.    phages carrying properties of plasmids
  4.    all of the above
 Discuss Question
Answer: Option A. -> combination of plasmid and phage λ
Answer: (a).combination of plasmid and phage λ
Question 15. M 13 is an example of
  1.    filamentous phage
  2.    single stranded DNA vector
  3.    both (a) and (b)
  4.    plasmid
 Discuss Question
Answer: Option C. -> both (a) and (b)
Answer: (c).both (a) and (b)
Question 16. Inserted DNA in λ gt 11 can be expressed as
  1.    β-galactosidase fused protein
  2.    free protein in the cytoplasm
  3.    free protein that is secreted out
  4.    all of the above
 Discuss Question
Answer: Option A. -> β-galactosidase fused protein
Answer: (a).β-galactosidase fused protein
Question 17. λ ZAP vector is an example of
  1.    phage
  2.    phagemid
  3.    cosmid
  4.    plasmid
 Discuss Question
Answer: Option B. -> phagemid
Answer: (b).phagemid
Question 18. Select the wrong statement about plasmids?
  1.    It is extrachromosomal
  2.    It is double stranded
  3.    Its replication depends upon host cell
  4.    It is closed and circular DNA
 Discuss Question
Answer: Option C. -> Its replication depends upon host cell
Answer: (c).Its replication depends upon host cell
Question 19. λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
  1.    6-7 kb
  2.    1-2 kb
  3.    40-44 kb
  4.    20-23 kb
 Discuss Question
Answer: Option A. -> 6-7 kb
Answer: (a).6-7 kb
Question 20. Stuffer is
  1.    the right arm of the vector DNA
  2.    the left arm of the vector DNA
  3.    central fragment of the vector DNA
  4.    none of the above
 Discuss Question
Answer: Option C. -> central fragment of the vector DNA
Answer: (c).central fragment of the vector DNA

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