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MCQs

Total Questions : 96 | Page 4 of 10 pages
Question 31. Cosmid vectors are
  1.    plasmids that contain fragment of λ DNA including the cos site
  2.    phages that lack cos site
  3.    plasmids that have no selection marker
  4.    cryptic plasmids
 Discuss Question
Answer: Option A. -> plasmids that contain fragment of λ DNA including the cos site
Answer: (a).plasmids that contain fragment of λ DNA including the cos site
Question 32. Maximum size of foreign DNA that can be inserted into a replacement vector is
  1.    25-30 kb
  2.    18-20 kb
  3.    20-25 kb
  4.    40-50 kb
 Discuss Question
Answer: Option C. -> 20-25 kb
Question 33. Difference between λ gt 10 and λ gt 11 vectors is that
  1.    λ gt 11 is a replacement vector
  2.    λ gt 11 is an expression vector
  3.    λ gt 10 is an expression vector
  4.    λ gt 10 is a replacement vector
 Discuss Question
Answer: Option B. -> λ gt 11 is an expression vector
Question 34. Which of the following is not true about phagemid?
  1.    Contain λ att site
  2.    May be propagated as a plasmid or as phage in appropriate strain
  3.    Can only be propagated as phage
  4.    Contain functional origin of replication of the plasmid and λ phage
 Discuss Question
Answer: Option C. -> Can only be propagated as phage
Question 35. PBR 322 has/have which of the following selection marker(s)?
  1.    Kanr
  2.    Ampr
  3.    Tetr
  4.    Both (a) and (b)
 Discuss Question
Answer: Option D. -> Both (a) and (b)
Question 36. A plasmid can be considered as a suitable cloning vector if
  1.    it can be readily isolated from the cells
  2.    insertion of foreign DNA does not alter its replication properties
  3.    All of these
  4.    it possesses a single restriction site for one or more restriction enzymes
 Discuss Question
Answer: Option C. -> All of these
Question 37. λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
  1.    40-44 kb
  2.    6-7 kb
  3.    20-23 kb
  4.    1-2 kb
 Discuss Question
Answer: Option B. -> 6-7 kb
Question 38. Conjugative plasmids
  1.    exhibit antibiotic resistance
  2.    do not carry transfer genes
  3.    carry transfer genes called the tra genes
  4.    do not exhibit antibiotic resistance
 Discuss Question
Answer: Option C. -> carry transfer genes called the tra genes
Question 39. Select the wrong statement about plasmids?
  1.    It is closed and circular DNA
  2.    It is extrachromosomal
  3.    It is double stranded
  4.    Its replication depends upon host cell
 Discuss Question
Answer: Option D. -> Its replication depends upon host cell
Question 40. Stuffer is
  1.    the left arm of the vector DNA
  2.    the right arm of the vector DNA
  3.    central fragment of the vector DNA
  4.    None of these
 Discuss Question
Answer: Option C. -> central fragment of the vector DNA

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