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MCQs

Total Questions : 96 | Page 7 of 10 pages
Question 61. Cosmid vectors are
  1.    cryptic plasmids
  2.    phages that lack cos site
  3.    plasmids that contain fragment of λ DNA including the cos site
  4.    plasmids that have no selection marker
 Discuss Question
Answer: Option C. -> plasmids that contain fragment of λ DNA including the cos site
Question 62. Cosmids lack
  1.    cleavage site for the insertion of foreign DNA
  2.    marker genes coding for replication
  3.    genes coding for viral proteins
  4.    origin of replication
 Discuss Question
Answer: Option C. -> genes coding for viral proteins
Question 63.  Maximum size of foreign DNA that can be inserted into a replacement vector is
  1.    25-30 kb
  2.    18-20 kb
  3.    20-25 kb
  4.    40-50 kb
 Discuss Question
Answer: Option C. -> 20-25 kb
Question 64.  A plasmid can be considered as a suitable cloning vector if
  1.    it can be readily isolated from the cells
  2.    it possesses a single restriction site for one or more restriction enzymes
  3.    insertion of foreign DNA does not alter its replication properties
  4.    All of the above
 Discuss Question
Answer: Option D. -> All of the above
Question 65.  pBR 322 has/have which of the following selection marker(s)?
  1.    Ampr
  2.    Tetr
  3.    Both (a) and (b)
  4.    Kanr
 Discuss Question
Answer: Option C. -> Both (a) and (b)
Question 66.  Which of the following is not true about phagemid?
  1.    Contain functional origin of replication of the plasmid and λ phage
  2.    May be propagated as a plasmid or as phage in appropriate strain
  3.    Contain λ att site
  4.    Can only be propagated as phage
 Discuss Question
Answer: Option D. -> Can only be propagated as phage
Question 67.  λ ZAP vector is an example of
  1.    phage
  2.    phagemid
  3.    cosmid
  4.    plasmid
 Discuss Question
Answer: Option B. -> phagemid
Question 68.  Difference between λ gt 10 and λ gt 11 vectors is that
  1.    λ gt 11 is an expression vector
  2.    λ gt 10 is an expression vector
  3.    λ gt 10 is a replacement vector
  4.    λ gt 11 is a replacement vector
 Discuss Question
Answer: Option A. -> λ gt 11 is an expression vector
Question 69.  λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
  1.    6-7 kb
  2.    1-2 kb
  3.    40-44 kb
  4.    20-23 kb
 Discuss Question
Answer: Option A. -> 6-7 kb
Question 70.  Select the wrong statement about plasmids?
  1.    It is extrachromosomal
  2.    It is double stranded
  3.    Its replication depends upon host cell
  4.    It is closed and circular DNA
 Discuss Question
Answer: Option C. -> Its replication depends upon host cell

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