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MCQs

Total Questions : 77 | Page 6 of 8 pages
Question 51. Maltose binding protein is the product of ______ gene in E.coli and located in ______
  1.    malE, nucleus
  2.    malD, nucleus
  3.    malE, periplasmic space
  4.    malD, periplasmic space
 Discuss Question
Answer: Option C. -> malE, periplasmic space
Answer: (c).malE, periplasmic space
Question 52. Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.
  1.    calcium ions
  2.    nickel ions
  3.    iron ions
  4.    fluorine ions
 Discuss Question
Answer: Option B. -> nickel ions
Answer: (b).nickel ions
Question 53. Thioredexin protein contains two _______ residues.
  1.    cysteine
  2.    cystine
  3.    adenine
  4.    guanine
 Discuss Question
Answer: Option A. -> cysteine
Answer: (a).cysteine
Question 54. His tagged proteins can be eluted using EDTA or a pH gradient from the matrix.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't Say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 55. Pel B protein is produced in plants and helps in the degradation of ______
  1.    vacuole
  2.    plasma membrane
  3.    cell wall
  4.    mitochondria
 Discuss Question
Answer: Option C. -> cell wall
Answer: (c).cell wall
Question 56. Expression of T7 promoter- lac operator hybrid requires ___________
  1.    T7 RNA polymerase
  2.    An inducer such as IPTG
  3.    Both T7 RNA polymerase and inducer such as IPTG
  4.    T7 DNA polymerase
 Discuss Question
Answer: Option C. -> Both T7 RNA polymerase and inducer such as IPTG
Answer: (c).Both T7 RNA polymerase and inducer such as IPTG
Question 57. A short peptide region fused to a protein of interest is known as ___________
  1.    tag
  2.    oligonucleotide
  3.    fragment
  4.    dimer
 Discuss Question
Answer: Option A. -> tag
Answer: (a).tag
Question 58. There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation.
  1.    solubility, phosphodiester bond formation
  2.    insolubility, phosphodiester bond formation
  3.    solubility, disulphide bond formation
  4.    insolubility, disulphidebond formation
 Discuss Question
Answer: Option C. -> solubility, disulphide bond formation
Answer: (c).solubility, disulphide bond formation
Question 59. When lacUV5 control system is used, addition of IPTG _______ the expression of _________
  1.    activates, T7 RNA polymerase
  2.    inactivates, T7 RNA polymerase
  3.    activates, T7 DNA polymerase
  4.    inactivates, T7 DNA polymerase
 Discuss Question
Answer: Option A. -> activates, T7 RNA polymerase
Answer: (a).activates, T7 RNA polymerase
Question 60. Glutathione-S-Transferase (GST) enzyme is used for the conjunction of Glutathione molecules and is having a protective function in many organisms.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True

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