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MCQs

Total Questions : 77 | Page 2 of 8 pages
Question 11. If detection of enhancers is done it is called as ______ and for detection of transcribed sequences, it is called as ______
  1.    enhancer trapping, promoter trapping
  2.    enhancer trapping, gene trapping
  3.    promoter trapping, gene trapping
  4.    promoter trapping, enhancer trapping
 Discuss Question
Answer: Option B. -> enhancer trapping, gene trapping
Answer: (b).enhancer trapping, gene trapping
Question 12. The enhancer trap system will _____ in the ______
  1.    not replicate independently, host
  2.    replicate independently, host
  3.    not replicate independently, vector
  4.    replicate independently, vector
 Discuss Question
Answer: Option A. -> not replicate independently, host
Answer: (a).not replicate independently, host
Question 13. Insertion of vector into intron gives rise to ________ it contains a part coming from intron and a part from ______
  1.    hybrid intron, exon
  2.    hybrid vector, exon
  3.    hybrid intron, target DNA sequence
  4.    hybrid vector, target DNA sequence
 Discuss Question
Answer: Option C. -> hybrid intron, target DNA sequence
Answer: (c).hybrid intron, target DNA sequence
Question 14. The reporter gene in enhancer trap system is preceded by ______
  1.    intron
  2.    exon
  3.    promoter
  4.    origin of replication
 Discuss Question
Answer: Option A. -> intron
Answer: (a).intron
Question 15. For gene trap vector system, reporter gene and _____ are present. Reporter gene is _____
  1.    selectable marker, having promoter
  2.    selectable marker, promoterless
  3.    helper phage, promoterless
  4.    helper phage, having promoter
 Discuss Question
Answer: Option B. -> selectable marker, promoterless
Answer: (b).selectable marker, promoterless
Question 16. Choose the correct statement for rifampcin.
  1.    It inactivates both E. coli polymerase and T7 polymerase
  2.    It activates both E. coli polymerase and T7 polymerase
  3.    It inhibits T7 polymerase but doesn’t inhibits E. coli polymerase
  4.    It inhibits E. coli polymerase but doesn’t inhibits T7 polymerase
 Discuss Question
Answer: Option D. -> It inhibits E. coli polymerase but doesn’t inhibits T7 polymerase
Answer: (d).It inhibits E. coli polymerase but doesn’t inhibits T7 polymerase
Question 17. The tac promoter is made by _____ region of trp promoter and _____ region of the lacUV5 promoter.
  1.    10, 35
  2.    35, 10
  3.    10, 10
  4.    35, 35
 Discuss Question
Answer: Option B. -> 35, 10
Answer: (b).35, 10
Question 18. Tac promoter is an example of which type of promoter?
  1.    hybrid promoter
  2.    fusion promoter
  3.    lacZ promoter
  4.    araBAD promoter
 Discuss Question
Answer: Option A. -> hybrid promoter
Answer: (a).hybrid promoter
Question 19. The ______ operon encodes proteins involved in arabinose metabolism.
  1.    araBCD
  2.    araABD
  3.    araBAD
  4.    araDBA
 Discuss Question
Answer: Option C. -> araBAD
Answer: (c).araBAD
Question 20. The tac promoter includes the lac operator and is regulated by a repressor.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True

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