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MCQs

Total Questions : 77 | Page 5 of 8 pages
Question 41. In ________ transcription and translation is coupled.
  1.    prokaryotes
  2.    eukaryotes
  3.    both prokaryotes and eukaryotes
  4.    both prokaryotes and eukaryotes, but favourable in eukaryotes
 Discuss Question
Answer: Option A. -> prokaryotes
Answer: (a).prokaryotes
Question 42. Ribosome binding site in E.coli is composed of which sequence primarily?
  1.    -GAGG-
  2.    -CTCC-
  3.    -ATGG-
  4.    -CGTT-
 Discuss Question
Answer: Option A. -> -GAGG-
Answer: (a).-GAGG-
Question 43. In which of the following systems, transcription and translation are carried out together?
  1.    Reticuloycte lysate
  2.    Wheat gram extract
  3.    Both in reticulocyte lysate and wheat gram extract
  4.    S-30 extract
 Discuss Question
Answer: Option D. -> S-30 extract
Answer: (d).S-30 extract
Question 44. Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.
  1.    ACG
  2.    AUG
  3.    UAC
  4.    CAG
 Discuss Question
Answer: Option B. -> AUG
Answer: (b).AUG
Question 45. If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.
  1.    few, translation
  2.    few, transformation
  3.    no, transformation
  4.    no, translation
 Discuss Question
Answer: Option C. -> no, transformation
Answer: (c).no, transformation
Question 46. Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.
  1.    True
  2.    False
  3.    May be True or False
  4.    Can't say
 Discuss Question
Answer: Option A. -> True
Answer: (a).True
Question 47. Which of the following is present in E. coli rrnB operon for rRNA?
  1.    Initiation codon
  2.    Termination codon
  3.    Both initiation and termination codon
  4.    Both are absent
 Discuss Question
Answer: Option C. -> Both initiation and termination codon
Answer: (c).Both initiation and termination codon
Question 48. Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________
  1.    hybrid protein
  2.    fusion protein
  3.    combination protein
  4.    insert protein
 Discuss Question
Answer: Option B. -> fusion protein
Answer: (b).fusion protein
Question 49. If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.
  1.    N terminal
  2.    C terminal
  3.    Both N & C terminal
  4.    Middle
 Discuss Question
Answer: Option A. -> N terminal
Answer: (a).N terminal
Question 50. It is also possible to generate a fusion protein in which ______ is encoded by the vector.
  1.    N terminal
  2.    C terminal
  3.    Both N and C terminal
  4.    Middle region
 Discuss Question
Answer: Option B. -> C terminal
Answer: (b).C terminal

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